Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 809 results for: TIM;

Showing: 441 - 460 of 809 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

  2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...editing, but when is the best time to shut off Cas activity? Using inhibitor timing experiments, the Doudna ... Additional Resources on the Addgene Blog First time CRISPR user? Check out this post. Learn how to Choose...

  3. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...types of people both above and below you. During my time at the NPA, there were many changes to the  Board... As I spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee...research field should I decide to remain here.  In a time when it is so important for postdocs and graduate...

  4. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags For about...ideal.  Unexpected connections Right around the same time that Munro and Pelham were devising their tagging...Schüchner, et al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...systems include that virus production can be quite time consuming and that insect cells require demanding...proteins from plants however can be expensive and time consuming as plant tissues themselves are biochemically...cells and growing conditions often need to be optimized. Yeast cells are also known for hyperglycosylating...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role...high-throughput genetic screen, it is expensive and time consuming to individually generate gRNAs and homology...the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...conference After using your elevator pitch several times at the conference, you might notice some trends....out if you’d like to meet someone and set aside a time to meet. Some conferences may also have a dedicated...there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour) and use the conference hashtag...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation, in...encouraging cells to grow and divide only at the right times and in the right places. Checks and balances: protecting...undergo programmed cell death while simultaneously stimulating growth and division. The combined effect is unregulated...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...cells as the virus. Because it is made at the same time as the other components of the vector particle, ...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...

  10. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... increasing HDR efficiency to read more about optimizing this distance. Homology arms and types of donors...apoptosis in as little as 10 hours, so read up on the timing of this approach carefully (Lin et al., eLife)!...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014) 10.7554/...

  11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...foreign DNA, and the Cas9-gRNA RNP is degraded over time, using RNPs may limit the potential for off-target... Cas9-gRNA RNP will be degraded by the cell over time. As a result, RNP mediated CRISPR experiments may...B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1 (2017...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...colony PCR can accomplish the same thing in less time and for less money. The key steps to colony PCR ... on a gel   Now that your PCR is complete, it’s time to run the products on an agarose gel to determine... biology tool kit. Consider giving it a try next time you’re screening for positive clones! Tips and ...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...advantage over conventional fluorescent dyes of the time was the fact that it was non-toxic and could be ... internal ribosome entry site (IRES). This is oftentimes used in conjuction with FACS (see below). Fluorescence-activated...protein to use in your upcoming experiments. In the meantime, if you haven't checked out Gal's blog, you should...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...teams succeeded with this approach around the same time (He et al., 2024; Tong et al., 2024; Ye et al., ...to mitochondrial base editing to base editors optimized for particular species and more, base editors ... X. (2024). Protein language models-assisted optimization of a uracil-N-glycosylase variant enables programmable...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...certain number of days and tracking average transit time for each courier we contracted with so we can always...navigated customs and arrived at their destination in a timely manner and at the correct temperature. To date,...written resources are valuable, there does come a time when a picture is worth a thousand words, especially...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Addgene repository can save researchers significant time and removes a variable from the lengthy process ... patterns so different? It could be a matter of timing, as jGCaMP7 was released relatively soon after ...informed choice on which AAV to request and/or save time in selecting experimental conditions. Sharing our...

  17. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

  18. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...authors) are currently working on improving and optimizing ARGs, with Marjorie focused on bacterial ARGs...imaging (Fig. 2d). Creating a cell line can be a time-intensive endeavor. That is why we also included...Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...can have low affinity for these tags and even multimeric tags are frequently insufficient for detection...Fluorescent proteins such as GFP have been used for a long time as probes to study connectomics (how neural circuits...microscopy. Sample preparation for these techniques sometimes makes it more difficult to detect protein tags...

  20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...uploading your Sanger sequencing files, either one at a time or as a batch of hundreds, and indicate the guide...do the rest. There are no parameters that need optimizing and no complicated steps to learn. For increased... can compare up to five editing experiments at a time, and batch analysis, which compares hundreds of ...
Showing: 441 - 460 of 809 results