We narrowed to 746 results for: TRI

...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...

...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...embargoed while the research is ongoing, but all registries do require that research plans become public ...

...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...

...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...

...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering... science, these tools sometimes have limited distribution. We hope that will change with Addgene’s expansion...

...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...gene or in multiple genes, all of which seem to contribute to a particular disease state. You could use ...

...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules...

...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular... depend on fluorescence intensity or protein distribution. Therefore, photostability measurements can ...

...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

...This post was contributed by guest blogger, Gary McDowell, executive director of Future of Research. ...salary raises at institutions will likely have a detrimental effect on the morale of postdocs, and indeed ...

...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...