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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: TRI

Showing: 441 - 460 of 738 results

  2. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you... mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...

  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering... science, these tools sometimes have limited distribution. We hope that will change with Addgene’s expansion...

  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...gene or in multiple genes, all of which seem to contribute to a particular disease state. You could use ...

  8. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

  10. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...

  11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...

  13. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules...

  14. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular... depend on fluorescence intensity or protein distribution. Therefore, photostability measurements can ...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...
Showing: 441 - 460 of 738 results