We narrowed to 530 results for: abo.2

...company culture.  It’s also a good idea to prepare 1-2 unique questions for each interviewer by looking at...you do to get ready for this interview? Maybe the above scenario sounds familiar to you. It was my experience...positions often required a one-hour presentation about a prior research project. In total, the interviews... find lists that have lots of general questions about job responsibilities, performance evaluations, professional...require. Frequently they will ask you to present about your prior research. This is a chance to highlight...

... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...blog Learn about the advantages and disadvantages of using monoclonal antibodies. Read about other techniques... require two different antibodies. As mentioned above, it can also be used to characterize antibodies ...concentration of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins...

...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

...inside the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor expressed in the human...students as we have done in the past. Below, read about the five winners and their research.   Abhi Aggarwal...work has only been made possible due to the collaborative and highly interactive environment that Janelia...biosensors or biosensors that detect new redox metabolites are vital for researchers to better understand...

...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...blog Find our CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender cell...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...custom design their own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning,...

...take care when using this method.      Fig 2:  A, The polyhistidine tag is a string of histidine...that this blog has answered all of your questions about polyhistidine tags. For more antibody-related information...

...protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral...drawback of this approach is that it can be quite labor intensive. Target cells are transduced with serial...

...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are... I heard about supervisors in all of the above categories. In addition, I also learned about two other...Complainers, who spend most of their time moaning about their troubles, Silent or unresponsive clams, ... students and postdoctoral fellows are hesitant about expressing their ideas, especially if their supervisors...it is interfering with your work. Avoid talking about your emotions, such as frustration or anger, because... like the problem to be resolved You know more about your work than your supervisor does. You can save...Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor might...

...unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...by the worm community. For additional resources about C. elegans and the plasmids we have at Addgene check...

...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...Sainsbury Laboratory. She is passionate about science communications and loves talking about science, ...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...break into science communication? Everybody goes about this in their own way. The career paths into science...Society gives training in media skills, writing about your research and residential communication. If ...

...non-specific binding in our IHC condition.   Fig. 2: Schematic of the goldilocks antibody selection process...Have you ever been stuck googling about an antibody that will be essential to your new research project...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and provide general...pattern should align with what the atlases (mentioned above) and literature suggest, providing an expectation...open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation by allowing scientists...

...systems to defend against both DNA and RNA viruses - 2 cas systems regulate at the DNA level and 1 at the... 2015 conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids... with the first keynote presentation. He talked about the impact of the Human Genome project on its 25th...of the meeting was how much we still don’t know about RNA. One talk that really brought this home was ...presented by Todd Lowe from UCSC. Dr. Lowe talked about his work on the 500+ human tRNA genes and, until... Udi Qimron from Tel Aviv University then spoke about using CRISPR/cas to address the growing clinical...

...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...Until then, you can only wonder what I am talking about. How do researchers use physical models of protein...students work closely with local researchers to learn about their research while creating physical models of...model is an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog...will wake up in the middle of the night dreaming about the conformational gymnastics that this protein ...Additional Resources on the Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips ...

... already have an end product in mind, steps 1 and 2 will generally be straightforward. Likewise for step...directly back to your specific goal. When thinking about step 3, however, you may be surprised at the number...Delivery methods for mammalian cell lines As mentioned above, the expression system you choose in many ways dictates.... The user should review the current literature about their preferred model.  If your expression system... Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (RNPs) ...

.... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...allowed gene circuits to be deployed out of the laboratory in a cell-free and biosafe format that was stable...circuit-based sensors to be used outside of the laboratory. The detection threshold for these RNA sequences...sequences (3 nM), however, was quite high and above what would be expected in patient samples (e.g.: blood...

... to remove this default setting. Timesaving Tip #2: blastx searches are inherently slower than blastn...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...mismatches and gaps in the alignment. If you are curious about the differences in the blastn programs, check out...Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources on...

...C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...protein activity while also capturing information about spatial and temporal organization. The design use...

...dimerization-dependent protein interactions. FEBS letters, 591 2, 415-424. PubMed PMID: 28032891. This is a different...Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in vivo...

...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs), Xue Han...