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TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...
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Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m.... of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB, brings ...host organism, the expression of some genes can change depending on the location. Current methods for ... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the Boards... Jennifer Tsang... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon You! Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed PMID: 16547094...16547094. PubMed Central PMCID: PMC1526508. 2. H. Jiang, et al. Cytokine/Jak/Stat signaling mediates regeneration... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m.... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m.... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m.... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation.... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m.... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....Developmental Editor at F1000Research. With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m.... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m..... Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’...:703–706. https://doi.org/10.1177/34.6.3084626 Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the Addgene... Angela Abitua...