We narrowed to 812 results for: cha

...is placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within...If something happens to the worm that requires a change of tube, there’s no clean way to separate the contents...a separate worm model and tube model, we can interchange them easily as needed. In this case, the physical...

...longer Cas remains active in a cell, the greater chances there are for off-target edits to occur. Although...effects Prolonged Cas activity can increase the chance of off-target editing particularly after the majority...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

...three species are still greater than random chance. The chances of a promoter expressing in primate and human...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...publications. Bonus: papers have to be summarized in 280 characters! Using Twitter to find papers  You’ll need to...a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional...

...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...Plasmids Isolated from Lactic Acid Bacteria:Their Characteristics and Potential Applications in Biotechnology...

...physiological conditions. Shyu et al. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP...physiological conditions. Shyu et al. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155...-noise ratio. Kodama et al. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments...

...a DNA sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore...retroviral transduction efficiency by neutralizing the charge repulsion between virions and the cell surface,...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, ...interference (RNAi) is an RNA-mediated gene silencing mechanism. Scientists can express siRNAs to direct the cellular...that can reveal how genetic alterations lead to changes in phenotype. The plasmid comes with a 1.9 kb stuffer...

...NGS in 2017 for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

...by the general public (either through taxes or charitable donations) so there is an increasing realization...funding or not; applications for funding from charities, commercial organizations, or universities may...-doc at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

...will be greatly reduced, allowing for clearer characterization of the location, morphology, and circuit mapping...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

... protein aggregation, you may want to consider changing your incubation temperature. Some proteins aggregate...kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane size is probably the easiest troubleshooting...alcohol. The increased SDS will increase the negative charge on the protein, allowing it to move more readily...Posttranslational modifications Check literature Highly charged amino acids Run control protein (if available...

... about his/her background. This will give you a chance to determine how to tailor your presentation so...sought out your poster, but will also give you a chance to slow down and catch your breath before diving... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...

... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...

... be translated at the ribosome into polypeptide chains that make up the primary structure of proteins.... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

...xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing PAM... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

...individuals in your team you will have a much better chance of creating a high-performing team - which is every.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly...

...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org...

...gave me a place to go once a week to get recharged. The fun change of pace helped break up the negativity...BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and...