We narrowed to 872 results for: fer

...twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural...Insertion of the mini-transposon can occur in two different orientations, but is strongly biased towards T-RL...combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and the...integration efficiencies achieved with VchINT, at two different incubation temperatures. Data adapted from Vo ...

...denotes intersection and the – operator denotes set difference. That’s the intuition for how genome math helps...enhancers from step 2. See which ESCs die or differentiate Step 3 reveals which NANOG-related genes are...distinct enhancer disrupted. As our ESCs die and differentiate over time, we periodically use next-generation...Therefore if a gRNA drops or disappears over time, we infer that the enhancer it targets is a “pillar of function...by starting with all of the DHS sites from 125 different human cell types from the ENCODE Project. DHS ...

...viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify...single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start...to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start signal...transcription of negative strand RNA viruses.   References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus...

...human pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (...For labeling the plasma membrane, they tested different target sequences for improved selectivity and ...into the supernatant. Four plasmids containing different nanobodies (NB) were generated in pMAK:  pMAK461....1038/s41598-020-57500-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

...moving around to explore the environment has a different motivation (and brain areas associated) compared...addiction. Anxiety tests Rodents have a natural preference to stay in dark areas (protected) as opposed ...to brightly illuminated ones (risky). They also prefer to explore novel environments by initially moving...anxiety. On the other hand, the control group preferred staying in the dark compartment (Howerton at al...these in a  future blog post, so stay tuned!  References Alpár, Alán, et al. "Hypothalamic CNTF volume...

...limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that...for target recognition and cleavage). C2c2 (now referred to as Cas13a) is unique in terms of structure ...remainder of this blog post. Perhaps the biggest difference between Cas13a and Cas9 is that Cas13a binds ...SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus, genotype human DNA, identify...Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O., et al. "C2c2 is a single-component...

... to determine the pros and cons of using many different types of gRNA design software. When you are happy...inserted or deleted. Each lab will use a slightly different method to genotype their pups. Taking a toe, a...weeks old and use the ear tag method (creating different patterns of ear holes and using that material ...latest and greatest in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target...plasmid into zygotes." Development, growth & differentiation 56.1 (2014): 122-129. PubMed PMID: 24372541...

...interviews, or get several interviews but no job offers, is a failure to clearly articulate one's talents...to convince me that their search committee is different and needs to be told all of the nuances of the...technologies, i.e. video interviews. For point of reference, I submitted my applications all by snail mail...hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with your search...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...delivering scFvs that inhibit HIV.   References and Resources References: Ahmad ZA, Yeap SK, Ali AM, Ho WY...for high affinity scFvs. Bound phage are then proliferated by infecting bacteria for additional rounds ... high affinity binding. Bound phage are then proliferated by infecting bacteria for additional rounds ...science.3140379  Bradbury ARM, Sidhu S, Dübel S, McCafferty J (2011) Beyond natural antibodies: the power...

...labs. While many labs approach the situation differently, some adopting simple note-taking tools, others...functionalities with experiment records. Many different types of ELNs are available on the market today... comparison to paper-based record keeping, ELNs offer functionalities that are hard to imagine on paper...desired functionalities i.e. main priorities of different ELN features, ranging from whether respondents... allows you to assign specific permissions to different lab members to avoid any issues later on. Do you...

...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...advisable to generate multiple constructs with different insertions, since it is difficult to predict which...can follow him on twitter: @joachimgoedhart.   References Adjobo-Hermans, Merel JW, et al. "Real-time visualization...

...of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded guide RNA (gRNA...agrobacterium-mediated delivery. Summary While different in many of the specifics - promoters used, precise...mediated genome engineering in plants isn’t all that different from how it’s used in other systems. Luckily, ... don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov...of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol...

... Even low-frequency editing could make a huge difference in DMD, and it’s estimated that exon skipping...respectively. Although each study used slightly different methods, they each observed the same positive ...disease type C. Although the three DMD studies referenced here represent a great step forward for CRISPR...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and...treatments may also be closer than we might expect.   References 1. Kaiser J. CRISPR helps heal mice with muscular...

...bottom of this post for a basic synopsis of the differences amongst the Brainbow systems. Pre-Brainbow techniques...to the promoter will be expressed. This system differs from Brainbow-1.0 in that transient Cre recombination...needed to mark individual neurons, the system also suffered from a number of limitations. First, the imaging...controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to ...2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial expression...

...PiggyBac transposons, the Woltjen Lab compared different variants of the polycistronic cassettes and discovered...a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...is a new member of this class that works via a different mechanism - the PhoCl protein is irreversibly ... are other RFP variants available that may be preferred for their intended uses, they provide a well-defined...of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown ...defined domains can be swapped onto other proteins to confer functionality. In Narayan et al., they used this...

...Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details on the assay, including...however, a physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a ...on the membranes will then be exposed to a lysis buffer to release recombinant proteins directly onto the...then suspend a freshly harvested dose of cells (preferably in log phase) in blocking solution (around 108...thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage...

...“NCBI Site Preferences” box. Once on the Preferences page, navigate to “PubMed Preferences” and click ... that you know how to navigate to the PubMed Preferences and Filters pages, be sure to check out the other...

...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure... pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO hChR2(H134R...

...modified allele is selectable, it can be transferred to a different genetic background via P1 transduction...Figure 2). In the following sections, key steps that differ from traditional restriction enzyme cloning will...could be used to move the modification into a different background, if needed. This approach is best suited...science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced levels of...

...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition... Jennifer Tsang...