We narrowed to 884 results for: ica

...systems are available at Addgene! Chemically inducible promoters Chemically regulated promoters are among ...sensitive expression systems are typically less leaky than chemically induced promoters; they show near-zero...transcription is turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples...prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein...decreasing promoter leakiness.   Other examples of chemically induced promoters include positive inducible ... promoters only work in prokaryotic cells and typically only in the same or closely related species from...

...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program focused...BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer to pesticide... Dr. Natalie Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...and teachers to apply engineering principles to biological systems. Today, what started in Cambridge with...Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid summer internships...

...cost-prohibitive for many labs, and are often impractical for small projects. Most researchers instead ...involves splitting up a sample into thousands of physically isolated partitions (such as droplets or wells...Since there are many thousands of partitions, statistical modelling can be used to determine the actual..., does not require standard curves, and can theoretically detect a single target molecule in a sample....a gene of interest is one of the most common applications of precision genome editing, the remainder of...single molecule resolution of digital PCR allows classification of individual target molecules based on their...single wells of a 96-well plate without prior quantification. Comparatively, mismatch assays require between...

...red fluorescent proteins) allow simultaneous identification of more than one protein of interest. However... species (bottom panel in Fig. 1). Our results indicate that the tag protein may have an important influence...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...However, in some cases the fluorescent signal may be critical for the experiment. Since the fused protein could...fluorescence expressed in the neuropil conceals identification of opsin-expressing cells). For other things...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper...Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex...

...and success. Customer and technical support Our customer and technical service brands continue to ...to keep up with the demand with excellence and dedication. We don’t have worry or wonder if providing...projects allows us to support more new projects. Diversification is happening. The Addgene team will have some... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...we might do. I am grateful every day for their dedication, flexibility, innovative thinking, and openness...Addgenies support a high collaboration, high communication, high efficiency, high quality, low meeting,...about the Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to...

... microscopy. This application of AHA labeling is known as fluorescence noncanonical amino acid tagging...PhD students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this... symposium seeking inspiration to resolve the technical hurtle of monitoring protein turnover of specific...technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine (AHA). In the technique...DNA strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...interested in neurobiology.  She is passionate about communicating science to all audiences. Right now, she is ...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ..., 98% of TdTomato expressing cells were GFP+, indicating a robust, but specific system. T-DDOGs also successfully... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...Could Cas9-DOG be developed soon to allow genome modification only in a small subset of cells? Other proteins... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...

... work around this is to "domesticate" your fragment: PCR-based amplification can be used to create silent...and exciting applications, this system is an incredibly powerful tool for cloning complicated constructs...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...restriction sites that may not be amenable to "domestication", you might want to consider using an alternative...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

...very useful in the event that you will not be physically present at your poster at all times, so other... sections should be as concise as possible, communicating only the most important results to your audience...have the advantage of being able to verbally communicate your work, so you don’t need every little detail...readers can navigate your poster and follow the logical progression of your work, and you can accomplish...offers free poster templates, although most are vertical.  Large central box. Another commonly used and...presentation. Portrait. This style displays a poster vertically rather than horizontally. Instead of displaying.... Shapes. In this type of poster, written and graphical information are inserted into larger circles or...

...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...researchers to identify and isolate very specific biological targets. However, as popular and useful antibodies...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and accessible database for...antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are a relatively...a research assistant in lab of Zhuhao Wu at the Icahn School of Medicine at Mount Sinai.  References ...

...production and subsequent necessary modification of these chemical products. The flexible nature of MoClo... Gate cloning is MoClo. That’s because MoClo specifically refers to the organizational schema described...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...together. They can express one or more gene(s), and typically begin with a promoter and end with a terminator...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule...genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You...

...generated in vitro using synthetic genes that are typically expressed from a plasmid or from an integrated...cells, for antibody production and subsequent purification.  Academic laboratories and companies have already...share, making recombinant antibodies the most practical choice. Hybridomas are much tougher to ship, often... often lack sequence data, and may genetically drift over time. Antibodies made from animals are limited...the ease with which scientists can now improve genetically-encoded research tools, it is shocking that the...Begley CG, Ellis LM (2012) Raise standards for preclinical cancer research. Nature 483:531–533 . https:/...Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans ...

...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In... become very different due to a whole genome duplication and extensive rearrangements in the S. cerevisiae...overexpression vector. This plasmid contains origins of replication and selectable markers that allow students to...functional MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes...met deficiencies (manuscript in preparation), indicating that Met protein functions have been largely ... Students who participate in the course show significant gains in both content knowledge and research ... research into undergraduate courses and in communicating science to the public. You can find out more...

...Lacks - the African American woman whose cervical cancer cells became a mainstain of biological study without...advice on how to dive into new forms of science communication. Click here to listen to the interview with ...fossil rocks and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully...implement the next generation science standards and communicate science. Learn more about Tom on his youtube ...

...example, if you’re a virologist presenting at the American Association for Cancer Research Annual Meeting...therapeutic strategies to target it, than you typically would at a microbiology meeting. Create an outline...lab mates, and PI can provide feedback on your technical presentation, but those outside of your field ...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought... through the list at the session so you can strategically use your time. Don’t have access to a list of...titles to find the ones you’d like to revisit. Strategically traverse the sea of posters If you come prepared...system, and is interested in a career in science communication.       Resources on the Addgene blog Read other...

...recognized by its antibody without additional chemical modification steps and when the epitope was within the...antibodies for every potential new target and basically impossible if you wanted to distinguish between...C-terminus post-translationally, which turned out to be critical for antibody recognition. As such, the peptide...interest and proteins tagged with it needed to be chemically treated before the antibody could detect them... the N-terminal half of the antigen differs significantly between human and the other species, suggesting... flexible - it could now be used in way more applications.   Fig. 1 Making a Protein Tag (Abridged... of new versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions ...

... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...stability, and buffer compatibility have placed practical limits on single- or two-step assemblies. We have...upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of the coding sequence... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings... sequence-dependent ligation efficiency and identification of mismatch-prone pairings. Using these 4 bp...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...

... the pharmaceutical industry for years. They have proven very difficult to target therapeutically due ...al. "Systematic identification of signaling pathways with potential to confer anticancer drug resistance... millions of people die from cancer-related complications each year. Despite these grim statistics, new... cancers, and synthesizing compounds to bind specifically to their mutated residues. For Ras researchers...any of these genes through several different applications such as fluorescent protein fusion studies and...represented, making the results of any such study as clinically relevant as possible. Connecting the research...

...another method for making targeted modifications. A recent publication by Zhang et al. shows just that -...about a month and a half, without the need for complicated cloning of homology arms. MMEJ: An Introduction...required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for knock-ins... and 3’ junctions, respectively. These results indicate that PITCh is a robust method for genomic insertion...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015...633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei...

...transformative biological discoveries and life-saving medical breakthroughs and undermine critical research ...Addgene as a trusted partner for archiving their biological materials during funding transitions. Enabling...operating model allows researchers to access authenticated materials at a fraction of the cost of creating...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...private partners to create additional pathways for critical scientific work to continue.  Moving Forward ...