We narrowed to 462 results for: invs
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TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...
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Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....results in effective gene delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....homology directed repair, these methods do not involve double strand break (DSB) formation and do not ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....environment itself. At Addgene we hope that all parties involved in making academic research a successful and enjoyable... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms that are ... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....factors to consider. First, the titer required is inversely related to transduction efficacy: the higher the... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....during our quality control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....then the result of their recombination is the inversion of the DNA between them (Fig. 1A below). In the... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....base of former mentees, we encourage them to get involved and give back by becoming mentors to new students... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....remains exposed for a longer time. The aerosols are invisible to naked eyes and hence cannot be detected until... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual colonies... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....- it is hard to distinguish between specific, invariable expression and non-specific, background signal... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m.... non-scientists alike, need to stay active and involved if we wish to make positive change. Below I present... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....were able to extravasate from the bloodstream to invade lymph nodes and scan antigen presenting cells, ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...