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We narrowed to 811 results for: lars

Showing: 441 - 460 of 811 results

  1. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...More recently, online journal clubs have become popular, including those based on Twitter. At my former...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    .... Standard curves are critical for a number of popular scientific applications, and the quality of a standard...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...produce your own recombinant antibodies with some molecular biology and cell culture experience. Let’s go ...further processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell...cells, and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing...Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism...unethical manner, but I also tried not to be an alarmist.  The overarching guideline for all of these ...thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of Chenery Middle School for their tremendous guidance...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...consisting of dCas9 and targeting gRNA, to identify molecular interactions at this site (Fujita and Fujii, 2013...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...inappropriately expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is...29480524. Gilbert, Luke A., et al. "CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...lab bench, we will know that a particular barcode and therefore particular sample is missing so that we ...information in a small space and is ideal for tracking large collections. Many companies, such as Thermo Fisher...preparation, and analysis from our protocols and molecular biology reference pages! Resources Avaliable on...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ..., mid- to late-career grant professionals have similar stories about a time when their passion for an ...was asked if I would consider consulting for a particular grant by another state. With the blessing of ...professional development. Do you want to work in a particular area, e.g. education, poverty, environment, early... Associates have secured more than $70 million dollars in grant funding. Jo serves on the Board of the...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...  Physical titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital...generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...). However, because purified AAV preps contain similar and low levels of background protein and chemical...email us and we can do our best to send you a particular lot (if we still have it available). References...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... the symptoms of mesothelioma are usually very similar to the symptoms of other diseases, such as asthma...diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates...with new ways to discover the cancer earlier, in particular before it starts to spread beyond its point of...tumors. Studies have shown that bevacizumab is particularly effective when used in conjunction with standard...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...Going Deeper: Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry...difficult. The first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette...experiments, simply induce injected engineered cells via regular intraperitoneal doxycycline injections to enable...

  11. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team...

  12. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse instead of...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The...mitochondrial base editing to base editors optimized for particular species and more, base editors are getting smaller... programmable T-to-G and T-to-C base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...luminescence wavelength (460 nm) is shorter than many popular fluorescence markers like GFP (510 nm), allowing...Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... interfering with downstream assays. This is particularly advantageous if the antibody used for IP is ... and structure. Their porous surface provides a large area for binding, but antibodies conjugated within...despite the size difference. Magnetic beads are particularly advantageous because they do not require centrifugation...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...and debris. The side scatter roughly measures granularity, a proxy for nucleic complexity of the cell.  ...antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These will be used in a series...they did in their experiments and determine if a similar approach would be good for your work. Hopefully...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best ...off-target cutting patterns in genome-wide screens similar to patterns seen in single-guide studies? Many ... Short gRNAs have lower off-target cutting and similar on-targeting cutting as full-length gRNAs Lastly...gold standards for human functional genomics." Molecular systems biology 10.7 (2014): 733. Pubmed PMID:...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...transcription factors, therefore promoters play a large role in determining where and when your gene of ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...Repressible Gets turned off with high levels of cellular tryptophan. lac General expression Promoter ...

  18. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...

  19. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ..., are limited by reality – a government cannot declare that gravity change direction and make things fly...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate... supporting it.  3) Linking scientists to one particular political party while alienating the other While...or politicians to interpret it for them. Other popular reasons to march include: General inclusion of...

  20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...29169465.  Ge, Yun, et al. "Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions...smaller biotin ligase for BioID proximity labeling. Molecular biology of the cell, 27(8), 1188-1196. PubMed ...
Showing: 441 - 460 of 811 results