We narrowed to 715 results for: mak

...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...

...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...

...has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited...

...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

...spectrophotometry. Microvolume spectrophotometers make it quick and easy to measure sample concentration...absorbance strength is low compared to other reagents, making it more difficult to detect. A particularly strong...

...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

... a specific field. While searching for jobs and making connections, avoid falling into an identity trap...yourself, and to demonstrate them to the people who make hiring decisions. Though having all the technical...

...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

...excited because genome-editing with these molecules makes it much easier to knock-in LexA binding elements...developing several additional systems of enChIP to make the technology more user-friendly and precise. We...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...effective reporter for adenine base editors, that make A-to-G edits in the genome (Gaudelli et al., 2017...

...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

.... Consultants often work with such teams to help make decisions about R&D investments in either new internal...benefits of novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running...

...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...