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We narrowed to 847 results for: ple

Showing: 441 - 460 of 847 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Using genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA...speed across these different cell types. In one example, H2B-FT was expressed in murine B cells (BaF3),... the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) ...

  2. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT... the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting out with... nicking the newly synthesized strand before heteroduplex resolution, which can lead to indels (Anzalone...edited strand as a template during DNA repair. Test multiple nick sites, starting with sites about 50 bp upstream...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... the mutation or replacing the gene. A notable example is the treatment of Severe Combined Immune Deficiency...normally functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies against ...: 14566358.  2. Hirsch, M. L., et al. "Self-complementary AAV mediates gene targeting and enhances endonuclease...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...bit of advice I can suggest, is to give yourself plenty of time when writing a fellowship proposal. Yes... you learn new techniques and interact with new people. If you are looking to move to a different university...there is an increasing realization that, if we want people to keep contributing to science, it would be helpful...audience. My lay paragraph typically includes a couple of sentences of introduction to my area, a line...great information on ELISA troubleshooting and multiplex assays.   Additional Resources on the Addgene ...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...challenges and opportunities for us all.  For example, implementation and compliance for the FLSA changes, including...turn. The first is the passing and upcoming implementation of the Federal Labor Standards Act (FLSA) which...remain mixed in their opinions on the subject – for example, Yale graduate students filed for union elections...challenging, but will be aided by the development and implementation of new strategies designed to cope with current...

  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...titers measure the amount of viral particles in a sample and are usually based on the presence of a viral...however, is that a p24 ELISA measures all p24 in the sample regardless of whether or not it is incorporated...measures infectious viral particles. One of the si mplest methods of measuring functional titer uses FACS...integrated into its genome and a cell that has had multiple integration events. In addition, promoter choice... cell’s genome. This approach can discriminate multiple integration events, reducing the possibility of...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...I knew when I first became a manager. Know your people Each person is unique and should be managed accordingly...accordingly.  I wish I knew that when I was “managing” a couple of undergrads back in grad school. Both were hard-working...Myers-Briggs test:  my undergrads were different people! More specifically, they had different learning... saying “I’ve been doing some of X’s work for a couple of weeks now”. I had no idea this was happening... and maybe even the slight awkwardness can spur people to speak up. Once aware of the problem, I was able...

  8. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...localized to a specific region of the cell. For example, if the ratio of red/green fluorescence falls within...experimental setup less cumbersome. 3. Portability across multiple systems Terskikh et al. showed that their versatile...in gene expression. In a developing embryo, for example, a researcher could determine whether or not newly...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...criticism, and ask thought-provoking questions. For example, if you’re a virologist presenting at the American...like to say to help build your presentation. For example, when thinking about discussing a figure, jot down...practice, practice: Rehearse your presentation with people inside and outside your field. Your colleagues,...brainstorm potential questions you may receive. People often ask about the relationship of your work to...guilty of this, and have many times answered a completely different question than the intended one. Take...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many...detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological... your research! If they do (or if they don't!), please consider sharing your experience with other researchers...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from... Multiple Authors...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...specific type of virus you are trying to make. For example, bacteriophages will need to be produced in bacteria...cells. However, some viruses can be produced in multiple different cell types, including plants, mammals...supernatant is typically collected over the course of a couple days to increase yield. The collection process ...preps, lentiviral titration requires an additional couple of days, as it requires transduction of more cells...titrated following purification using qPCR or digital droplet PCR. Insect cells Insect cells are a less common...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...like “Oh,…I didn’t quite appreciate that….” For example, when Tom Steitz was presented with a physical ... close to its reported cleavage site on the complementary DNA strand. And this is why we love working ...double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target...

  13. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... plasmid page is nested in. You can see in the example above that the bread crumbs trail starts in the...  These sequences are provided in FASTA format (example) and can be analyzed using Addgene’s Analyze Sequence...Price of course! The price of some items vary, for example, plasmid kits are avaliable for less than than ...which experimental systems it was validated in.   Please keep in mind that all of the information on our...ever have any questions about our plasmid pages, please don't hesitate to contact us at [email protected]...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen, 1992) because of their ... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...beginning to realize that the situation is already complex enough that a deep understanding of retroviral ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions...Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological Reviews, 56(3), 375...

  15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and ... not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is ...lots of individual cells will be transduced with multiple viral particles and labeled a variety of colors...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...unprepared. Each year in the United States, two million people will be infected by antibiotic resistant bacteria...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

  17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...with the viral polymerase, which is formed by a complex of two proteins: the large (L) catalytic protein...-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as ...efficiently reconstitute a functional viral polymerase complex and utilize the minigenome as a template. Thus,...transfected into cells are also extremely important. For example, particular ratios of plasmids encoding NP and ...mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ... a few time-tested tags and fusion proteins as examples. The tables list more common tags than are described...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

  19. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...citations so that people can go back and read the original reference for the data. For example, we summarized...sense of the themes in the field and to learn what people cared about. I also used reviews to get a list ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...paraphrase what I have heard many other creative people say about writing: you don’t know what it’s going...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism,...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...babble as baby birds, and have dialects just like people. This is actually rare in the animal kingdom. Gasterosteus... most grown crop in the world so it feeds a lot people. This lab is trying to improve homologous recombination...
Showing: 441 - 460 of 847 results