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We narrowed to 772 results for: INA

Showing: 231 - 240 of 772 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...washing and then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with a PCR...fragment size and (2)  incubate with RNAse and Proteinase K to purify the DNA and then determine concentration...isolated your antibody:protein:DNA complexes and finally (5) isolated the DNA from your antibody:protein... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...expression and largely defines the amount of recombinant protein that will be produced. Many common promoters...Positive inducible In the OFF state, the promoter is inactive because the activator protein cannot bind. After...Negative inducible In the OFF state, the promoter is inactive because a bound repressor protein actively prevents...tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response elements...a reporter gene. When light is present, YFI is inactive, preventing repressor cI synthesis and allowing...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... the synthetic biology scene in 1989 through a seminal paper from Peter Schultz’s lab describing a technique...same for all three components, meaning that the final protocol may involve a sensitive balance of trade-offs...GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin board on their website...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...their target protein is altered by blue light illumination. This ability to reversibly bind and release...tagged, which limits these approaches to use with recombinant proteins. Purifying an untagged protein with ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...pathway. What’s next for OptoNBs? While OptoNBs illuminate a path to light-controlled protein binding, they...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...any type of DNA or RNA purification to avoid contamination, take extra care when performing RNA extraction...using DEPC-treated water, autoclave the water to inactivate the DEPC.  Ensure that plasticware or glassware... baked at 180°C for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...resuspended RNA sample will help ensure DNA contamination is not an issue.  References                 ...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...have higher reproducibility and consistency in the final product. Because the constant (non-epitope binding...reduced immunogenicity. Effector functions are eliminated, as is antibody recognition, since antibody-specific...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific...rapid means of comparing functionality of N- and C-terminal fusions and degradation in a cell line of interest...express ENL-FKBP12F36V and endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules...express FKBP12F36V-MELK and endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules...

  8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...expressing the recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated...the number of clones that do not contain the recombinant plasmid and therefore makes the cloning process... but should never be used when selecting for recombinant plasmids. Check out our blog post on Common Lab...References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” Biotechniques. 1996 Aug;21(2):320...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...solution depended on the specific antibody:target combination of interest (Figure 1). It can therefore be helpful...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...within the five pillars and is frequently used in combination with other approaches. If several cell lines ...correlation with other approaches when possible. Finally, correlation of -omics data can also be used in... 2019, 2021). In those studies, we used this combination to find suitable antibodies for a target with...

  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...every sweet or toy strewn across the floor. In the final wake it all settles down and the value of each item...of research activity and development since its inaugural year in 2012. That’s just 3 to 4 years ago. It...problems various tools are trying to solve, and finally how we might proceed in the future. When we think...for sgRNA design (by Hsu et al., 2013) used a combination of both empirical and approximate methods. The...online”, “CLI”, and “GUI”. Trends: Online tools dominate the software space but generally rely on less ... active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology (2014). PubMed PMID: ...
Showing: 231 - 240 of 772 results