We narrowed to 821 results for: LAS

...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ...articles (3/12/2014). With so many options for CRISPR plasmid tools and numerous experimental design decisions...with expression, though it is possible for the plasmid to still express without the G. Because the G is...experiments for multiplexed targeting with Zhang lab plasmids? A6: For multiplexing CRISPR to target multiple...into PX330, then co-transfect or delivery the two plasmids together into your cells. If you want to use double...very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them, and...donor template, it's usually more efficient than plasmid donor. Typical design is to have 50–80 bp of homology...

...our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression...AAV and lentiviral preps we have released in the last few months: Lentiviral preps for expressing SARS-CoV...protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning.... Now, we've turned some of the most requested plasmids into lentiviral preps to speed up COVID-19 research...Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO...constructs that allow for targeted expression. Plasmid 75470 is now available in AAV1 for Flp-dependent... mCherry reporter. We now also offer AAVrg for plasmid 83898, which targets expression of ChR2 to forebrain...

...virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural genes...crossover event between the vector plasmid and the packaging plasmid caused the Psi sequence to be placed...expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your gene of...usually 293T cells, with your transfer plasmid and your packaging plasmids. For this, you can use the transfection...techniques are still used and efficiently transfer plasmid DNA efficiently into dividing immortalized cells... (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert of interest as well as sequences...the packaging signal Psi). Because the packaging plasmids lacked the psi signal, the genes encoding the ...

...science project was to make my own glass milk for purifying cut plasmid DNA from agar slices.  Molecular...me that most Addgenies make an effort to avoid plastic bags and to conserve resources where possible.  ....  At the lunch table, for instance, we have a plastic container problem…reusable containers pile up by...sink after lunch as fewer and fewer people use plastic bags.  We also try to recycle our discards according...about conserving resources in the labs. Disposable plastic materials are fairly inexpensive in this country... funded and we didn’t feel the need to reuse plasticware or think about conserving reagents and buffers...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  These...

...are excited by the yellow/green laser but only "bright" for the blue laser. To understand why that is, let's...brightness changes to "dim" for the same laser type. Knowing that the red laser excites at 640 nm (Table 1), while...separately detect these markers in your analysis. Lastly, there are fixable dye stains that react with free...few commonly used fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design...-fluochrome-brightness-spillover Excitation Laser Color Very Bright Bright Moderate Dim ... brightness beyond the fluorophore itself, like laser type and power, acquisition voltage, the concentration... let's look at Figure 2. The blue laser excites at a wavelength of 488 nm (Table 1), which is near a local...

...as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging...The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR...repair template. SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent...tag and homology arms). Digestion of the donor plasmids with SapI allows the tag, selectable marker, and...to be released (Fig. 1, Step 2-3). Since donor plasmids of the same type will produce the same unique ...different combinations of connector and tag donor plasmids from the library can be used to generate functionally... of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker ...

...While the bulk of these plasmid deposits came in this year, there were some plasmids deposited as early as... We started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in ...the scientific community the latest and greatest plasmid tools! Thank you depositors! In fact, this year...quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been...the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve’s lab as an example). Depositor... of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your science along...and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of ...

...more stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific...commonly performed by transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains...chosen for both Cas9 and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target...targets with similar efficiency as compared to plasmid-based expression of Cas9/gRNA and can be used for... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...fluorophore tagged dCas9 may require the use of plasmid or viral-mediated delivery. You’ve decided to...and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab...

...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...MORF Collection) and an accompanying expression atlas, including over 3,500 human TF isoforms, to help...understand this regulatory network. The transcription atlas and library The transcription factor library The...determine how its presence influences cell fates. The atlas of directed differentiation To understand how each... fates. (Adapted from Joung et al.)    This atlas linked TF expression to differentiation outcomes...characterized TFs, the Zhang group were able to classify unknown, or ‘orphan’, TFs into their respective...cell types generated by the human fetal expression atlas. Computational analysis was used to infer differentiation...

... 1998). Interested in plasmids that contain the groucho gene? Check out plasmids deposited by the Shivdasani... several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however was... to see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman...website for plasmids that contain tinman or the mammalian homolog Nkx2.5. We also have one plasmid, Addgene...Drosophila Read our Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research ...the stories behind the names. We also have many plasmids in our repository that you can use to study these... Beauty (Ivics et al., 1997). We have a lot of plasmids that contain components of the SB transposon system...