We narrowed to 611 results for: STO;

...site. Recipient cells express DsRed within a lox-STOP-lox sequence, followed by the EGFP, and the TEV ...translocates to the nuclease, and excises the DsRed-STOP to trigger expression of EGFP. Figure 2:... MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023). The power and potential of mitochondria...

...and whose structures are just beginning to be understood. Therefore, in addition to their utility in the... strain that you can use for long-term library storage. After overnight growth, scrape up, pool and extract...during one of these steps is also a convenient stopping (ie. going home and eating and sleeping) point...

...adapt. By the end of my three months, I fully understood why parasitology roles in the field require experience... available at a small science non-profit doing customer service (recruitment agencies often don’t tell...I was also surprised to learn that I enjoy the customer service side of my job. Because of my background...

...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

...Despite its relatively recent development, the history of optogenetics begins almost 50 years ago with...Archaea, the third in green algae (Oesterhelt and Stoeckenius, 1971; Matsuno-Yagi and Mukohata, 1977; Foster...PMID: 22480664. Oesterhelt, Dieter, and Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane...

... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein isolation, then the ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol...