...adapt. By the end of my three months, I fully understood why parasitology roles in the field require experience... available at a small science non-profit doing customer service (recruitment agencies often don’t tell...I was also surprised to learn that I enjoy the customer service side of my job. Because of my background...

...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

...Despite its relatively recent development, the history of optogenetics begins almost 50 years ago with...Archaea, the third in green algae (Oesterhelt and Stoeckenius, 1971; Matsuno-Yagi and Mukohata, 1977; Foster...PMID: 22480664. Oesterhelt, Dieter, and Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane...

...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein isolation, then the ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol...

...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

...leads to the first base transversion editor The story of base transversion editors starts with researchers...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing... newly synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post...