We narrowed to 884 results for: ica

...   Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary page with PubMed...result interpretation. The analytical method for score assignment and significance thresholds used by the ...favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section...new freely available resource developed by the Biological General Repository for Interaction Datasets (... to allow facile queries of published data by biomedical researchers and ready access to stably archived...manually curated and annotated from more than 45 publications, beginning with the seminal research articles...CRISPR screen results available to the entire biomedical research community, but also allows cross-interrogation...

...May 22, 2019 Smart Servier Medical Art This site has 3,000 free medical images organized into four main...software that let me create graphics that better communicated the science in my blog posts. This post highlights...free tools which will hopefully also help you communicate your science, whether it’s in presentations, ...number of drawing tools, so it’s harder to draw intricate figures, like a brain or a mouse. Vectr Vectr ... over 20,000 icons is designed by professional medical illustrators with input from life scientists. It...without the watermark, which are better suited for publications, if you sign up for a paid account.  I’m proud... anatomy and the human body, cellular biology, medical specialities, and general items. Individual images...

...Rep78/68 play a key role in AAV genome replication. Replication initiates from the AAV genome ITRs on ...Srivastava A (2019) Capsid Modifications for Targeting and Improving the Efficacy of AAV Vectors. Molecular...contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains the ...aka the RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type...its products. Created with BioRender.com   Replication (rep) gene The rep gene encodes four proteins...proteins that are required for viral genome replication and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene... domains, all four Rep proteins share a common helicase and ATPase domains. To function, the Rep proteins...

... tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (clustered...genes, which code for polymerases, nucleases, and helicases, were also disrupted to elucidate their role in...destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA...distinct types and subtypes using polythetic classification based on evolutionary similarity (5). Type ...2013 (7, 8). Nine months later, more than 1500 publications describe work to improve the tool’s specificity...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,...programmable platform well-suited for various innovative biological and translative technologies. Addgene has empowered...

...Cell-Type-Specific Gene Editing." Journal of the American Chemical Society 140.21 (2018): 6596-6603. PubMed PMID...valuable to scientists, there are some potential complications that must be considered when using this method...alternative approach, which avoids many of these complications, is to directly deliver a ribonucleoprotein ...used for most of the current genome engineering applications of CRISPR including: generating single or multi-gene...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required... of eventually using this method for in vivo applications. This technique utilizes Cas9 proteins harboring...Liang et al. 2017).  PEG-mediated methods, while technically less difficult to perform, often show low efficiency...

...where you assume your technical skills define you and that the other not-so-technical skills you’ve built...my job. The same can be said about teamwork, communication, writing, management, and creativity; I have... individual with more going for you than your technical expertise in a specific field. While searching...built over time are only applicable to those positions you have already held. Imagine this situation: You...have the desired degree, you have mastered the applications they use, you’ve gained experience working with...under your belt from your last job. You knock the technical portion of the interview out of the park. It’s...hiring team will also want to examine not just technical skills, but also transferable skills, before offering...

...mismatches on modification by AsCpf1 (red) or LbCpf1 (blue). Mismatch positions are indicated below each ... this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification of CRISPR-Cas...it lacks Cas9’s other HNH endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...compared the ratio of total off-target to on-target modification for AsCpf1 and LbCpf1, and found that both orthologs...-target editing in a cell culture model. The application of Cpf1 to genome editing is exciting both in...

...require grant seekers to use online applications with significant character limits for the written portion...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant...the candidate application process, the candidate’s ability to write a grant application through a written... much more than writing. In fact, many grant applications and grant makers have moved away from long written...portion of the grant application. They have increased their demand for stronger budgets with clear budget...duties can range from coordinating the grant application and submitting it, to evaluating the capacity...especially in the sciences and by extension to scientifically oriented nonprofits. Like Deb, I worked for...

...dynamic, physiological processes. Re-engineering GFP to increase its range of color and application Almost...through mutagenesis in order to improve its physical and biochemical properties. In 1995, Roger Y. Tsien described...transgenic mice. Purification: GFP can be used as a general epitope tag for protein purification and a number...scientists to begin to study GFP and deduce its biochemical properties. Now GFP and its fluorescent derivatives...GFP is used in research across a vast array of biological disciplines and scientists employ GFP for a wide...their experimental needs, and some of the common applications in the lab. Why Green Fluorescent Protein?...others) Neutral mutations    A multitude of applications Due to its size and ease of use, GFP and other...

...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...advantage of a fusion protein is the efficient purification of an already immobilized target protein. The...immobilization method. One possibility is covalent modification of the purified target protein with the vitamin...derivatives streptavidin and neutravidin) is for all practical purposes covalent. Therefore, protein immobilization... four biotin-binding sites per molecule so, theoretically, saturated avidin could form tetramers of biotinylated...well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand...However, large amounts of the target protein are typically required due to the relatively low efficiency ...