... CRISPR, there's a pretty good reason for that. Lately, significant advances in the understanding and ...another very different bit of CRISPR news was circulating. Scientists at North Carolina State, in the labs...as an even more precise and useful tool. A week later, researchers at the Broad Institute and MIT published...

...incubation step and go straight to plating if your transformed cells are being plated on agar with ampicillin selection...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make sure ...

...that really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step...phages or with phages targeting organisms closely related to the clinical sample. For treatment, it is best...phages for clinical use. There are also different regulations from country to country when it comes to administering...

...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...to female. Then, it detaches from the gills and latches onto the base of the tongue where it pierces the...References Biron, D. G., et al. "Behavioural manipulation in a grasshopper harbouring hairworm: a proteomics...

.... With this information in hand, you can then formulate a plan to further develop your transferable skills... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...10.1016/j.molcel.2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong...

...on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The Addgene themed... intention of ending the celebration. The party later moved to State Park, where I had a great (maybe ...helped bring it to fruition. The concept, though relatively simple, changed the course of scientific research...

...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from.... In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing ...

...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...Multiple new site-specific recombinases for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda...Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 ...

... be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments, just about...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to... husband Enzo will spend a few days editing and later looking for songs and sounds to synchronize with...

...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (... once bound to an antibody, can act as immune modulators, shaping the immune response by driving particular...Presta LG, Ravetch JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat...

...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

... far too long. Holding these meetings in relatively isolated resorts creates a sense of camaraderie with...Plasmodium falciparum. Throughout these talks, I oscillated between amazement at how much has been done with...

...aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes the ...

...software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these...control elements Coding sequence features are relatively straightforward to define, but for control elements...fluorescent proteins, which often come in closely related versions that have different properties. To prevent...

...access to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant...vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids ...

...launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral preps to...all of your data prepared. All of these questions relate to factors that you should have already recorded...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

...cargo, they can’t reproduce more functional viruses later on. These built-in safety features provide extra...integration resulting from transduction typically plateaus within a few days to a few weeks, depending on...host nucleus, is transcribed into RNA, and is translated into protein.   Is there an easier way? In...

...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

...plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...integrated into the chromosome” was proposed a few years later by Élie Jacob and François Wollman and became the...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...