We narrowed to 509 results for: Cit
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TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....might help formulate some hypotheses. They are excited to see other researchers from a range of fields...
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Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....residues. For Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2].... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....molecular biology of synaptic transmission and plasticity. Javier García-Nafría is an investigator ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....their associated ancestries. While there were some exciting relationships identified, 48% were found to be... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....your event in casual conversation to get people excited! Laura Veckerelli is the Administrative and... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J mice is likely due to how the Deverman... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....your off hours. DIY bio labs are cropping up in cities around the world, such as BosLab here in Boston... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... of interest. The wide availability and high specificity of anti-epitope antibodies make this approach... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m.... B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized ... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....Partnership Management, a very interesting and exciting part of my work. However, this is not the first... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers...