We narrowed to 799 results for: Comp

... was also between 1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy...fluorescent voltage indicators, however, fall short compared to GFP-based sensors in terms of response and ...precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems...

...proteins within a system. This can be pretty complicated given that a single cell has tens of thousands...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...bacteriophage shell protein or a bacterial microcompartment protein. The expression of this fusion is ...

...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...Cas7-11 Cas7-11 is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains...

...Stanford University. Sex and gender are critical components of biological research that are often forgotten...cost at this early stage of research is minimal compared to that of human clinical trials. And, as Janine...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

...Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future members...more than two sequencing reactions. In the more complex cases, we sometimes have to design new primers ...saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...

...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust...strain can be rescued by the xanthine produced via complete demethylation of caffeine. For example, the Barrick...a 1.2-fold greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. ...

...allowing us to do up to 96 transformations in one run compared to doing about 50 transformations in the same ...increased plasmid demand it was too time consuming to complete this process by hand and we welcomed yet another...liquid handler can fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in...

...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)...deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed ncRNA (...interactions between ncRNAs and adjacent genes are complex and not yet fully understood, so it is important...

...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

...get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor ...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

...cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted expression was...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three...

...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ... have involved turning on fetal hemoglobin to compensate for defects in the adult hemoglobin produced ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating...

...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...proteins interacting. Experiments tend to be equally complex with users trying to probe multiple targets in ...

...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

...share, remix, store, and track these essential components of the scientific toolbox. What are Recombinant...subsequent purification.  Academic laboratories and companies have already begun creating plasmids encoding ...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...

...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...

...who are usually antagonistic and impolite, Complainers, who spend most of their time moaning about their...with negative comments (hostile-aggressives, complainers, know-it all experts, wet-blanket negativists...support from your colleagues or other supervisors to complete your work. Whether your supervisor is a negative...