Skip to main content
Addgene
Showing: 461 - 480 of 620 results

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable with...use in yeast, as parts of biosenors, and much more. Do you have a favorite use for PA-FPs? Let us know in...

  6. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...

  7. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other types of ...

  14. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...
Showing: 461 - 480 of 620 results