We narrowed to 623 results for: Dos;
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TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....We began our research with a simple question: how do Cas9 and sgRNA interact with and dissociate from ...
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Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....think of entrepreneurship in the 21st century, who do you think of? Your mind is likely drawn to the Mark... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction sites that... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....would would make a good fit for me?" Whatever would I do next and what options lie ahead of me? Like a considerable... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable with...use in yeast, as parts of biosenors, and much more. Do you have a favorite use for PA-FPs? Let us know in... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m.... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m.... disillusioned with the gulf between what I was doing in the lab and the interactions I was having outside... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other types of ... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....the histogram plots look a bit different than they do for yes-no readouts. Figure 4: A histogram... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....You can find more information about us and what we do in our Frequently Asked Questions. We also invite... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....questions regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....majority of restriction enzymes commonly used in the lab do not have recognition sites that could overlap with... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...