We narrowed to 517 results for: EMB
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TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...
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Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....’t work for all bacteria. Zeocin Zeocin is a member of the bleomycin family of antibiotics, which induce... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m.... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m.... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....commitments, respond to any issues raised by team members, and plan for the future of the team. All while... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... Science Night, a chance for parents and other members of the community to share their expertise with ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced...