We narrowed to 520 results for: EMB

1. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

2. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ... We invite feedback and contributions from all members of the research community as we continue to to ...

3. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   Published

   Feb. 25, 2016, 3:30 p.m.

   ...positive. I went back into lab the next day and remembered why I still love science. Keep building on your...

4. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

6. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

7. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

8. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... Science Night, a chance for parents and other members of the community to share their expertise with ...

9. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

10. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

11. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

12. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

14. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

15. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

16. AAV Titers: Where Do They Come From And What Do They Mean?

    Type

    Blog Post

    Published

    Nov. 15, 2017, 3 p.m.

    ...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category...

17. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc...

18. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced...

19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

20. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...