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We narrowed to 681 results for: Eng;

Showing: 461 - 480 of 681 results

  1. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...to the right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...multiple genes by CRISPR/Cas-mediated genome engineering." Cell 153.4 (2013): 910-918. PubMed PMID: 23643243...Addgene Blog Get the Basics of Mouse Genome Engineering with CRISPR Validate Your Genome Edit Learn about...

  6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments. However,...problems of popular fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation...

  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...recombinant proteins are typically expressed in E. coli engineered to accomodate high protein loads using inducible...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...linking the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution...method, where the donor is excited by a specific wavelength of light and the signal is collected by using...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA ...1472-6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  16. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...

  19. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

  20. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...
Showing: 461 - 480 of 681 results