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We narrowed to 685 results for: Eng;

Showing: 461 - 480 of 685 results

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could also...://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ... showing frequency of events on the Y axis and strength of signal read on the X axis may be presented....

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes...are just so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details...

  6. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...Addgene blog Learn about what went into our search engine update Find all Addgene news Resources on Addgene.org... Sophia K. Cheng...

  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...to the right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...multiple genes by CRISPR/Cas-mediated genome engineering." Cell 153.4 (2013): 910-918. PubMed PMID: 23643243...Addgene Blog Get the Basics of Mouse Genome Engineering with CRISPR Validate Your Genome Edit Learn about...

  9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments. However,...problems of popular fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation...

  10. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...recombinant proteins are typically expressed in E. coli engineered to accomodate high protein loads using inducible...

  11. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...linking the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution...method, where the donor is excited by a specific wavelength of light and the signal is collected by using...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA ...1472-6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms...

  17. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  20. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...
Showing: 461 - 480 of 685 results