We narrowed to 530 results for: Erf

1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ... DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore fusions on...

2. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ... can be stored right away. Every week, the lab performs an average of 140 transformations. The lab typically...

3. Plasmids 101: Protein tags

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   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA GEGEIPAPLA...

4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification...

5. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every...

6. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...specified discrepancies. Any sequences that are a perfect match from this script can then continue in the...

7. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community...

8. Hot Plasmids Spring 2024

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   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

9. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   Published

   May 21, 2024, 1:30 p.m.

   ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

10. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...enough information there for you to be independent performing the technique you trained to do? Walk through...

11. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

13. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

14. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

15. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

16. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

18. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...you realize. Being at a university provides the perfect platform for practicing initiative and for training...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...