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We narrowed to 771 results for: INA

Showing: 461 - 480 of 771 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...Created with BioRender.com. In recent years, deaminase-mediated base editing (cytosine base editor or...FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking...their level of explanation in presenting data. Finally, let me take this opportunity to recommend all ...your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities to show cool visuals...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality... designed multiple gRNAs to target the 5’ long terminal repeat (LTR) of the HIV-1 genome, which acts as... Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells ...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to...repair to allow for detection, not Homologous Recombination or Alternative End Joining, two alternative ...different gRNA would have distinct off-target sites, eliminating its effectiveness as a control. Since off-targets...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this...thought to be reversible once the effector is inactivated/removed from the system. In contrast, epigenetic...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...transformation due to background re-circularization and contamination from uncut plasmid. 4. Transformation Transform... higher competency cells. Additionally, if your final product is going to be very large (>10kb) you might...DNA and lets other materials pass through; and, finally, selectively elute the plasmid DNA from the column...

  7. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...be analyzed with a paired t-test, because they eliminate subject-to-subject variation (the test assesses...tests exist to identify significant pairwise combinations – Tukey’s test, Newman-Keuls, Duncan’s multiple...Comparison of Multivariate Means across Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...

  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...

  11. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ... and quantify how new technologies are being disseminated through the scientific community. Data on CRISPR...technology was also greatly accelerated through a combination of open-source distributors such as Addgene, ...

  13. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation... Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A big thank you to all who have contributed...

  14. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...on the Addgene repository, are also attending! Finally, our team is rounded out with our science communication...conference experience on X, Instagram, and Bluesky.   Finally, if you're looking to brush up on your conference...

  15. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert adenosine to inosine. Activate/Repress...

  16. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...or lab manager’s approval before letting your imagination run loose on the lab. Have fun! Here’s an example...

  17. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

  18. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...we wouldn’t necessarily have expected when we originally sent out the call for applications. Cervical ...caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls...

  19. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...you would like us to feature in the coming year. Finally, we are always looking for guest bloggers who want...and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...

  20. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...insert. Example, if you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert...
Showing: 461 - 480 of 771 results