...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...other markers can be injected into an animal, to directly label circulating cells or other cells that are...

...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...intended application — here, flow cytometry — is the desired target, using one or more of the approaches outlined...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...

...decision. We realize that with this move, we lose a direct connection with our nearly 22,000 X followers. ...official Addgene account. You can reach out to us directly via email at [email protected], and we invite you...

...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D...

...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

...Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA or RNA sequences...Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular DNA or RNA sequences...

...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...

...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...postdocs various academic institutions can afford to hire. It will be difficult for some of these institutions...

...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard ...

...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological...PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists to continue to do excellent...

...insert are often denoted by "wt" either directly before or directly after the insert name.   Example: If ...

...Cambridge before spending time in biotech screening direct interactions between drugs and promoters of oncogenes...and extremophiles for biotechnology and has been directly involved in supervising many successful iGEM undergraduate...

... an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

...cups of coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is... a day, and I had colleagues who were much more wired. Here at #SfN14 there are thousands of posters being...

...target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is ...stand breaks and may better facilitate homology directed repair. You can find all of these variants, as...

...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early...

...sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition to this cis cleavage...Cas12 are for the other types of nucleic acids and required a certain sequence specificity in the middle of...