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We narrowed to 792 results for: LAT

Showing: 461 - 480 of 792 results

  1. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...LinkedIn. We are also exploring other social media platforms with active scientific communities and welcome...functionality and features that previously made the platform a mainstay of conversation in the scientific community...considering a gradual move off X as a response to major platform changes, such as limiting access and increased...

  2. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...cells. The goal is to use cells that can be easily isolated from donors. Apart from fibroblasts, human keratinocytes...epithelial cells from urine are some of the easily isolated somatic cells that have been reprogrammed to iPSCs...the reprogramming factors) More Chances of accumulation of harmful mutations More, due to selection ...from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci...

  3. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Abhi Aggarwal gets the reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

  4. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...the scientific community. Here are a few virus-related protocol videos that may help to save you time ...AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used to generate...with tissue cultures. To keep up on all of the latest protocol videos, lab tips, and more video content...

  5. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Früh (@Michelderapfel) December 19, 2022   Congratulations to all our #DeckTheLab contestants!           ...tubes, hang up your pipettes, and seal up those plates – it’s time to turn your attention to decorating...decorations and that you’re following all safety regulations. That’s one sticky situation our customer service...

  6. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...t cross the blood-brain barrier. By lowering circulating levels of kynurenine in the body, the brain is...Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  7. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... CRISPR, there's a pretty good reason for that. Lately, significant advances in the understanding and ...another very different bit of CRISPR news was circulating. Scientists at North Carolina State, in the labs...as an even more precise and useful tool. A week later, researchers at the Broad Institute and MIT published...

  8. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...incubation step and go straight to plating if your transformed cells are being plated on agar with ampicillin selection...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make sure ...

  9. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...that really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step...phages or with phages targeting organisms closely related to the clinical sample. For treatment, it is best...phages for clinical use. There are also different regulations from country to country when it comes to administering...

  10. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...to female. Then, it detaches from the gills and latches onto the base of the tongue where it pierces the...References Biron, D. G., et al. "Behavioural manipulation in a grasshopper harbouring hairworm: a proteomics...

  11. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    .... With this information in hand, you can then formulate a plan to further develop your transferable skills... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...10.1016/j.molcel.2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong...

  12. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The Addgene themed... intention of ending the celebration. The party later moved to State Park, where I had a great (maybe ...helped bring it to fruition. The concept, though relatively simple, changed the course of scientific research...

  13. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from.... In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing ...

  14. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...Multiple new site-specific recombinases for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda...Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 ...

  15. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments, just about...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to... husband Enzo will spend a few days editing and later looking for songs and sounds to synchronize with...

  16. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (... once bound to an antibody, can act as immune modulators, shaping the immune response by driving particular...Presta LG, Ravetch JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat...

  17. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

  19. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes the ...
Showing: 461 - 480 of 792 results