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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 708 results for: MAK;

Showing: 461 - 480 of 708 results

  1. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    .... Consultants often work with such teams to help make decisions about R&D investments in either new internal...benefits of novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...protein research institute, partnered with Addgene to make our collection of integrin antibodies available ...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... available as lentiviral plasmids if you want to make a stable cell line. This design allows highly localized...bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third...

  9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...alleles had unwanted insertions/deletions, therefore making those alleles useless for our studies. If one now...

  10. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... unmethylated DNA, and more. Many mutations that make these features possible are present in most commercial...commercial strains, especially mutations that make major improvements such as those that increase plasmid...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay attention...knocked down or knocked out. If you are interested in making your own knockout line for validation, please view...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... disease states, and targeting the epigenome may make certain processes, like cellular reprogramming of... likely cell type- and context-dependent, it may make sense to try multiple CRISPR tools when setting ...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...

  17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...the parental animal, are rescued progeny, which makes screening extremely rapid. This stringent selection...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...

  18. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...

  20. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...switch from a dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...
Showing: 461 - 480 of 708 results