We narrowed to 662 results for: MAL
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TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....assembly order of the fragments as compared to the smaller overlapping sequences left with a restriction digest...incubation times, or the ability to accommodate smaller fragments. Know your cloning methods There are ...
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Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m.... my expensive habits of running shoes and single malt whiskies, but I remember the days of modest postdoc... very hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with ... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen,...therefore consisted of glycines, serines and other small, non-aliphatic amino acids. However, long linkers... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m.... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m.... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes and... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....its target states are only represented in a very small number of registers. Instead, one may use qPCR to... not be suitable for applications that require minimal cellular perturbation. Also, this method requires... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments. Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....experiments. Biology is messy enough that by chance, some small number of the scientists will get a result that ...order to chase a positive result. Beyond these more formal systems, individual labs can certainly institute... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m.... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....societies, and industry through convening activities, formal lobbying, or strategy setting. The “little p” ...question may change over time and that’s perfectly normal! Action Items: Start a career journal during... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....Moreau (1896) where he explores the concepts of animal testing and modifications. The premise of the novel... novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... (22). Large purine:purine mismatches and most smaller pyrimidine:pyrimidine mismatches are typically ...screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....found that many researchers feel that they lack formal training in a variety of transferable skills. At...until the team really starts functioning (or malfunctioning). Clear lines of communication Few people actually... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself...