We narrowed to 666 results for: Mal

...fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen,...therefore consisted of glycines, serines and other small, non-aliphatic amino acids. However, long linkers...

... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands...

... my expensive habits of running shoes and single malt whiskies, but I remember the days of modest postdoc... very hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with ...

... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population...

... which are commonly used to drive expression of small RNAs in their respective species. Addgene carries...allow researchers to insert targeting oligos with minimal cloning required. As with other model systems, ...

... a crowd. Having answer slides ready after your formal conclusion slide will also help with your confidence...with other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular...

...assembly order of the fragments as compared to the smaller overlapping sequences left with a restriction digest...incubation times, or the ability to accommodate smaller fragments. Know your cloning methods There are ...

...populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes and...

...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less...

...experiments. Biology is messy enough that by chance, some small number of the scientists will get a result that ...order to chase a positive result. Beyond these more formal systems, individual labs can certainly institute...

...antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-...

...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments.  Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range...

...), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to...

...biomolecular condensate in human cells — causes a rare malformation syndrome (Mensah & Niskanen et al., 2023). Now...oxygen consumption and upregulating other genes normally expressed in BAT. When donor-derived CRISPRa-AAV-treated...

... frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell...

...in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:...

...its target states are only represented in a very small number of registers. Instead, one may use qPCR to... not be suitable for applications that require minimal cellular perturbation. Also, this method requires...

... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility...

...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself...

... (22). Large purine:purine mismatches and most smaller pyrimidine:pyrimidine mismatches are typically ...screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM...