We narrowed to 629 results for: Ncl
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TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well as a chimera (...
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Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....progress without it… These details often aren’t included in scientific communications, but they’re firmly... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....of those entering science communication (myself included) come from a scientific background and bring with... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m.... by any number of characteristics. Common ones include age range, familiarity with the subject, shared... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....Pfizer vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....get context, time, cause, effect, and a solid conclusion. Advice for Telling a Good Story Start at the... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....secondary is not binding endogenous IgGs, you should include no primary antibody controls in your experiments... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m.... with a caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....) is a process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....interest. Potential applications of the system include activation and repression of your target gene, ... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....need that free hydroxyl group! You may want to include extra extension time after the last cycle of PCR... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....number of unique features compared to Cas9. These include: More targeting flexibility Unlike Cas9 and the... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....similarly, taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....attacks can be brought on by a number of stressors, including emotional stress or changes in temperature. “... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m....used to make many other genome modifications, including targeted mutations. SEC-based systems may prove... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....said he hopes others will find his tools useful, including his zinc sensor and his genetically encoded magnesium... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....of interest into its component simple sugars (including lactose), and a lac operon controlled GFP. When... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....Basically, the CO2 goes in and the CO2 goes out. Conclusions and handling recommendations Not all O-rings ... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....Management. His research focuses on a range of topics including biotech and research tools, computing performance... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m.... To minimize these issues, researchers usually include a separate normalization step where individual ...