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We narrowed to 847 results for: PLE

Showing: 461 - 480 of 847 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism,...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...babble as baby birds, and have dialects just like people. This is actually rare in the animal kingdom. Gasterosteus... most grown crop in the world so it feeds a lot people. This lab is trying to improve homologous recombination...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...citations so that people can go back and read the original reference for the data. For example, we summarized...sense of the themes in the field and to learn what people cared about. I also used reviews to get a list ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...paraphrase what I have heard many other creative people say about writing: you don’t know what it’s going...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ....), while PE6c and PE6d were most efficient at complex edits, such as long or highly structured templates...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...tet-responsive element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...easy to share plasmids, both for people depositing plasmids and for people requesting them. Prior to this... and remove these logistical barriers, allowing people to access a wide catalog of plasmids from all over... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...manipulated for use in molecular biology studies. For example the E. coli biotin ligase, BirA, has been used ...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...abilities. The axolotl has the ability to regenerate complex structures such as limbs, the spinal cord, and ...them an ideal model organism; they are relatively simple to maintain and breed and they have large, easily... and microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...cells will be co-transduced with multiple copies of both vectors. Examples of use: Halbert et al., 2002...then following transduction, the partial genomes complement each other to restore the full-expression cassette...outperform single oversized AAVs (Hirsch et al, 2013). Examples of use: Lai et al., 2005 used a trans-splicing... the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a hybrid split...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for...trace file generated in Sanger sequencing and a simple algorithm to quantify editing efficiency by insertion...have to screen to identify a knockout clone. For example, if your cell line is diploid (2N) and your out...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso... Multiple Authors...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...protein phosphorylation in an array of model systems coupled with advances in mass spectrometry suggest that...recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA synthetase... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...are performing CRISPR library screens, the same principle holds true - the higher the number of distinct...

  10. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to... give you a fuller picture. Consider talking to people in the lab across the hall or in the same department...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible.... lab members alone during the interview period. People seem to be hiding their work and are nervous about...successful. Collaborative Competitive Papers have multiple authors One author plus PI on most papers Productive...

  11. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

  12. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...journey is at an end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted... name. So we reached out to you and received a plethora of options, ranging from Affie to Yncredibody....Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this...Original Blugene and Aavery would like your attention, please, as they introduce their new friend…  Fig...

  13. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ...cells are fused with activated B cells from the spleen of mice injected with the antigen of interest. ... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...

  14. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins that, in...Abdallah et al., 2018; Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors... Han, 2016). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could.... "A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit ...Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new...

  15. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...generate 2 possible products: 1) a partially dsDNA duplex with single-stranded 3’ overhangs or 2) if the ...dsDNA was short enough, a ssDNA whose entire complementary strand was degraded. Beta: Beta protects the...created by Exo and promotes its annealing to a complementary ssDNA target in the cell.  Only Beta expression...region of interest and design an oligo that is complementary to the lagging strand. In practice, it’s easier...system once recombinant clones are generated.  A simple way to do this is to express lambda red genes from...

  16. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...generated through your organization in this field. Please also enter the billing address for your organization...time, your organization will have net 30 days to complete payment for your order.  Still have questions?...

  17. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...competition is fierce. However, I believe there are plenty of great jobs out there for science PhDs. The problem...organizers are building valuable skills and meeting new people by working together on the event planning and execution... execution. I am seeing many similar examples of science trainees taking a proactive role in their non-bench... Consulting. I know of many schools that have completely student/postdoc-driven biotechnology clubs such...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...visualization in vivo using ultrasound (for an example see the figure below). In addition to low costs...benefits of this technique. It is even possible to multiplex ultrasound dyes because these dyes “collapse” ...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized approaches...gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon gap using gifs”:...

  19. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to droplet digital...Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might be that you can't...Purification Without a Kit by Beth Kenkel To complement our RNA Extraction Without A Kit blog post, we've...

  20. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t ...high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high throughput washing...lab culture, but with the right incentives (for example, by grant agencies), we can shift toward a more...more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips cost ...
Showing: 461 - 480 of 847 results