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We narrowed to 790 results for: Por

Showing: 461 - 480 of 790 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...belief that αVβ3 would be the most functionally important integrin on these cells. What’s next IPI is working...

  2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...split aptamer is composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence...photostable, and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO...growth as RNA biology, we are looking forward to reports on the next additions to aptamer soup! References...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...invasion and D loop formation, the newly synthesized portion of the invasive strand is displaced from the template...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  5. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...findings can be predictably applied to future work. Importantly, no one should expect that every study should...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...and use computer based analysis techniques, an important component of reproducibility is making sure that...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ... Despite the shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based...

  7. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...mycoplasma contamination The best and the most important ways to avoid mycoplasma are by providing proper...viability. Conclusion To sum up, it is always important to prevent contamination rather than eradicating...others when you can and help them understand the importance of preventing contamination. Be aware and cautious...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  9. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... adenovirus [5], Sendai virus [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]...insulin-producing β-like-cells [37, 38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...

  10. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... DNA to favor the edit Once the prime editor incorporates the edit into one strand, there’s a mismatch...MutSα–MutLα mismatch repair (MMR) complex, to temporarily inhibit this process and promote copying of the...it from hybridizing with the unedited strand. Temporarily inhibiting this complex may allow time for 5′...

  11. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    .... They also mutagenized the cytidine deaminase portion of the base editor to create SpCas9 base editors...off-target activity (Richter et al., 2020). This is important because ABEs have generally been slow, meaning...editing for plants Read about four base editor reporters Additional Resources on Addgene.org Catch up...

  12. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...the phage recombineering machinery are first electroporated with pCas9. Then, pCRISPR is introduced along... a mammalian cell line (Qi et al., 2013). Two important features of this early system are its regulatable...Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014) Exploiting...

  13. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...reflections on the program are here. This was a great opportunity for Addgene to present our own data on plasmid...in advancing this field and how we are able to support rapid progress in all areas of research. We welcome...

  14. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that...

  15. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...India, and Brazil.  We've also created a robust support system for scientists using materials in our repository...repository, from our customer and science support to our efficient and easy tech transfer process to our...

  17. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...Whenever possible, we love to give scientists the opportunity to share their knowledge. One of our goals is...social media outlets meaning you’ll have the opportunity to interact with and discuss your work with many...

  18. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...to meet the diverse needs of our community and support a wide range of experimental designs. Here are ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

  19. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use viral ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...
Showing: 461 - 480 of 790 results