We narrowed to 599 results for: REV;

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...or polymerization will occur at that position, preventing T4 from chewing back the entire 10–12 bases. ...

2. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...different from the yes-no histogram plot shown previously. Here, the orange peak is a baseline expression...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...components. Pol Precursor protein containing Reverse Transcriptase and Integrase components. Transfer...

4. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...

5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ... University School of Law, where she was on law review. She clerked for Juan R. Torruella, U.S. First ...

6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...survive (ex: antibiotic resistance marker). In a previous blog post, we covered how to use positive and ...

7. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ... more precise base editors capable of editing previously uneditable sequences. Four DNA base swaps, so...

8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...immunology PhD student at Harvard University. In my previous two posts, I’ve described the fundamentals of ...

9. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...enzymes that can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction...

10. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...detect NHEJ. These assays include a forward and reverse primer to amplify the target locus, as well as ...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ..., Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding behavior...

12. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...epigenetic editing. This area is discussed in our recent review.      And that's a wrap on our AAV webinar! Special...

13. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...visualized in a single experiment, which had not been previously possible. This advancement could be especially...

14. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them. You’ll also ...

15. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ... common antibody after IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus...

16. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version of the Cre-...

17. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...Careful documentation will keep you on task and will prevent you from getting lost in the wide word of informatics...

18. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...familiar territory, but quickly diverges from previously discussed methods. As described above, you design...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ... Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)    ...

20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a technology that was difficult...