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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 841 results for: RING

Showing: 461 - 480 of 841 results

  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...Addgene, I felt comfortable asking questions without fearing they would be disregarded - everyone was very approachable...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...

  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    .... If you decide not to cleave the GST tag after/during purification, be aware that the tag dimerizes in...purifications due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian ...possible but requires optimization and often more stringent washing. Nonetheless, this tag can be purified...

  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...H840A creates 3’ overhangs in a PAM-out design.  Exploring nickases for homology directed repair The potential... Mary Gearing...

  4. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

  5. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ..., had to keep him from running too much as any jarring of his body could initiate very painful FOP flare...donation via the link below. Please continue to help bring HOPE to AJ.   PS: The superhero race is the perfect...running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for the past ...

  6. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...obstacles that stand in the way of the doers and by ensuring good communication. A manager must both hire and...delegating tasks and in motivating their doers by ensuring they are as engaged as possible. To learn more...of leader, one needs to take the initiative by bringing and effecting solutions to both known and unidentified...

  7. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...associated genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and...system that scientists have harnessed for genome engineering. The diversity of the CRISPR/Cas systems provides...who has the strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome...

  8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...screens require a low multiplicity of infection (MOI) during cell library construction to ensure that the majority...screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical issues, ... proteins can bind specific DNA sequences which brings the attached fluorescent protein to the DNA. They...

  9. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...

  10. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...robust tools that have practical applications in monitoring the accidental or intentional release of engineered...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...

  11. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...Agreements, your TTO signs each agreement just once, covering nearly all future requests/deposits. This reduces...Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute...Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center  Monash University  Montana State...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...preventing them from moving forward? Answering these questions will bring you and your audience - via the ...sense of curiosity and wonder why you’re doing and sharing science in the first place?   References and Resources...

  14. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...syndrome, a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The...competition for researchers working on rare diseases, offering them the chance to win some of the latest tools...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ...how to sell these products to other scientists. Tailoring my CV for the job To become an attractive candidate...removed my techniques and protocols, courses taken during my PhD and Undergraduate sections and brought in...

  16. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...identify potential career pathways, education and mastering new skill sets must occur by doing work outside...can: Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...Networking As the number of qualified scientists entering the workforce increases, EAs allow graduate students...

  17. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

  19. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex... the run time is just a few minutes per sample. During the run, DNA fragments move through a micro- or...

  20. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    .... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...
Showing: 461 - 480 of 841 results