We narrowed to 512 results for: SET

1. Science Careers: Unruly Interests Feed Many Paths

   Type

   Blog Post

   Published

   Sept. 26, 2017, 12:12 p.m.

   ...interests, though, have proven unruly. Instead of settling down into a singular focus on molecular pathways...

2. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   Published

   March 1, 2018, 2:11 p.m.

   ...volume that’s compatible with your experimental setup. Some experiments require higher gene expression...

3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

4. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ... In 2012, Casey Maguire and colleagues from Massachusetts General Hospital and Harvard Medical School ...

5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   Published

   April 7, 2022, 1:15 p.m.

   ...you can use to express the iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction...

6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   Published

   Feb. 13, 2024, 2:15 p.m.

   ... as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can...

7. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

8. Addgene Depositors Get More Citations

   Type

   Blog Post

   Published

   April 20, 2017, 2:30 p.m.

   ...numbers. Note: this does drop some journals from our dataset, since not all journals have papers with plasmids...

9. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

10. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which they showed also...

11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...Transformation of Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed...

12. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...vectors Of the many viruses out there, only a small subset are commonly used as tools in the lab. We will ...

13. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...

14. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ... tailored image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian...

15. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...Partitions that did not contain any target DNA at the onset of PCR are negative, whereas those that did are ...

16. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

17. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

19. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

20. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...