We narrowed to 497 results for: SUB
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TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...
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Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m.... Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution of enrichment... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m.... the General Meeting seven other times I had subsequently changed fields and my cohort of friends and ... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....quencher from the proximity of the fluorophore and subsequently leading to a positive signal. The same effect... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....decision determines whether the client invests substantial monetary and human resources towards the development... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m.... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m.... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...