We narrowed to 490 results for: SUB

1. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...

2. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...quencher from the proximity of the fluorophore and subsequently leading to a positive signal. The same effect...

3. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... the General Meeting seven other times I had subsequently changed fields and my cohort of friends and ...

4. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...

5. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...

6. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

7. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example...

8. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...

9. Changing Labor Laws Bring Increased Postdoc Wages

   Type

   Blog Post

   Published

   Oct. 25, 2016, 2:30 p.m.

   ...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example...

10. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...decision determines whether the client invests substantial monetary and human resources towards the development...

11. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    Published

    Nov. 5, 2014, 3:41 p.m.

    ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

12. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

13. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done...

14. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...

15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...

16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...

17. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...

18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

19. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing...

20. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...