We narrowed to 495 results for: SUB;

1. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...

2. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

3. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... the General Meeting seven other times I had subsequently changed fields and my cohort of friends and ...

4. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

5. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example...

6. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...quencher from the proximity of the fluorophore and subsequently leading to a positive signal. The same effect...

7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...

8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ... Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution of enrichment...

9. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity...

10. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii...

11. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example...

12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...decision determines whether the client invests substantial monetary and human resources towards the development...

13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which...

14. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...

15. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    Published

    Nov. 5, 2014, 3:41 p.m.

    ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

16. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

17. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done...

18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...

19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...

20. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...