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...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...