We narrowed to 526 results for: She

1. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

2. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

3. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

7. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...

8. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

9. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

10. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

11. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

12. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

14. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

15. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

16. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

17. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

19. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...might help to get you started. Once you have established a well working protocol in your lab, it can become...

20. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....