We narrowed to 813 results for: TIM;

...can have low affinity for these tags and even multimeric tags are frequently insufficient for detection...Fluorescent proteins such as GFP have been used for a long time as probes to study connectomics (how neural circuits...microscopy. Sample preparation for these techniques sometimes makes it more difficult to detect protein tags...

...uploading your Sanger sequencing files, either one at a time or as a batch of hundreds, and indicate the guide...do the rest. There are no parameters that need optimizing and no complicated steps to learn. For increased... can compare up to five editing experiments at a time, and batch analysis, which compares hundreds of ...

... generating mutants. Unexpectedly, phenotypes sometimes differ depending on whether a gene is deactivated... a high-resolution map of early embryogenesis in time and space, and was also used to precisely characterize...redundant) genome, their long three-month generation time and labor-intensive maintenance compared to invertebrate...

...choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity concerns...variety of Cas9 containing plasmids that have been optimized for expression in different species and cell types...delivered Cas9 and a sgRNA to your target cells, it is time to confirm that your target sequence has been modified...

...to serve as a primer for DNA repair synthesis, ultimately leading to the formation of double Holliday junctions...Challenges: Standardization and HDR CrispyCrunch Optimizing Donor DNA for Enhanced CRISPR Genome Editing ...Methods in Enzymology Vol. 546; (2015). PubMed. Optimization of Genome Engineering Approaches with the CRISPR...

...therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed et al...re-solubilize and re-fold scFvs from inclusion bodies, it’s time-consuming and laborious.  To avoid this, one or ...https://doi.org/10.3390/antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design...

... videos for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker...was out the window. So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If ...everyone through the recording process was going to be time consuming and difficult. Finding volunteers who ...

...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491... al. "Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of...29472486. 17. Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation...

.... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...manufacturers’ PCR protocol. Be sure to adjust the extension time to match the size of your template. Amplification...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...

...tweets from the Symposium under #FORBOS15) - my first time live tweeting: a fun and surprisingly exhausting...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ... is certainly true that not all PIs can take the time to do this, you should always reach out to your ...

... IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary antibodies...antibody may be binding non-specifically – try optimizing your blocking and washing conditions or consider...-reactivity. Keep in mind that you may need to optimize the conditions and concentrations of all the different...

...same pace and enter the resolving gel at the same time where they are then separated by size. Gradient ...concentrations, blocking buffers, and incubation times. If the vendor includes publications that cited ...   Analyzing the western blot Once visible, it’s time to analyze your protein of interest. Analysis typically...

...that sometimes it is necessary or desirable to control or look at multiple genes at the same time.  The...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...

...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...mentee’s personality and professional needs at any time. In its Pilot Program 2014-15, IMP provided mentorship... monthly progress video conferences and had real-time correspondence via e-mail throughout the entire ...

...silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved, the...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ... manually extracted. This process is tedious and time consuming, as RNAi mutants with a reduced growth...

...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...switching between different colored ligands at different time points.   Figure 3: Single-molecule tracking...

...of protein function. In addition, the level and timing of expression can also be affected by the tag.  ...immune complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...health and limit debris, keep samples on ice at all times and use gentle pipetting instead of vortexing. Include...

...a large majority of neuronal cells are labeled overtime due to the continued replication of the virus....have been widely used by neuroscientists for some time due to their advantageous characteristics for brain... titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5, AAV8, ...

...It’s a very thankful time of year for those of us at Addgene! And we can’t think of any better way to...Addgene blog? We'd love to hear from you! Email us anytime at [email protected]. ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ....G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses the VSV-...