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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 745 results for: TRI

Showing: 461 - 480 of 745 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...

  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    .... These plasmids have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  10. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... to prep large quantities of DNA because some countries just can’t receive bacteria through the mail due..., if you have any great lab automation tips and tricks, be sure to share them in the comments section....

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...

  14. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

  16. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...license. If you’re currently using mVenus or mCitrine, or if existing yellow fluorescent proteins just...anterograde labeling across single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...genes for drug discovery or to understand the intricacies of molecular pathways. These screens have gotten...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca... academic lab is closely involved with clinical trials or has ties to industry, the drug discovery process...
Showing: 461 - 480 of 745 results