We narrowed to 484 results for: Vit

1. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated...

2. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

3. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...

4. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...

5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...

6. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you may need to optimize the...

7. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...by Cas does not always correlate with catalytic activity at those sites, especially when DNA mismatches...

8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ...regulation, as long as they can control recombinase activity. For example, post-transcriptional recombinase...

9. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...repeats (ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful...

10. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

11. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...have meetings between teams. This communication is vital because otherwise I’d lose touch with my colleagues...

12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln...

13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low levels...

14. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...When you are hunting for labs to join, one of the vital things to look for is a supportive environment—it...

15. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...

16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...particularly interested in science communication and in vitro diagnostics.     References 1. Costantino N, Court...

17. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ... clinic regulations, evidence-based research is vital in combating misinformation and propaganda that ...

18. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...sequences can be optimized for specificity and sensitivity, for example by changing certain amino acids ...

19. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...called CasPEDIA, with information about enzyme activity, experimental conditions, and more. Type II Type...

20. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured cells with a virus that...