We narrowed to 524 results for: emb

...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

... We invite feedback and contributions from all members of the research community as we continue to to ...

...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

...But we hope this guide can help you get started. Remember, you can always change your compensation values...

...positive. I went back into lab the next day and remembered why I still love science. Keep building on your...

...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

... Science Night, a chance for parents and other members of the community to share their expertise with ...

...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...