We narrowed to 520 results for: emb
-
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....But we hope this guide can help you get started. Remember, you can always change your compensation values...
-
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....positive. I went back into lab the next day and remembered why I still love science. Keep building on your... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m.... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m.... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....’t work for all bacteria. Zeocin Zeocin is a member of the bleomycin family of antibiotics, which induce... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... Science Night, a chance for parents and other members of the community to share their expertise with ... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....commitments, respond to any issues raised by team members, and plan for the future of the team. All while... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...