We narrowed to 517 results for: emb

1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

3. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

5. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

6. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

7. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... Science Night, a chance for parents and other members of the community to share their expertise with ...

8. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

9. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

10. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

11. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...

12. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

13. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

14. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

16. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus...

18. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

19. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

20. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...