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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 842 results for: ins;

Showing: 461 - 480 of 842 results

  1. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...show frustration if they're incorrect; take it instead as valuable feedback.  Listen. Think about the ...strategy. Don’t assume it’s the audience’s fault; instead look for ways to improve how you're communicating...If something I was communicating didn’t click, instead of blaming the audience, I would think about how... wasn't. Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...for every assay. Antibodies that are generated against a linear antigen tend to work very well for applications...application. Take immunohistochemistry (IHC), for instance. While an antibody may be validated for IHC, not...region highly conserved across species? If the proteins are highly homologous between your target species... each isotype or species to easily discern the proteins in your assay.  Narrowing the field After working...compare the staining patterns of multiple antibodies against the same target; tagged protein strategies that...

  3. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...destination vector + insert may spontaneously religate, this transient construct retains functional Type IIS...in the final construct. The destination vector contains sites with complementary overhangs that direct...complete with helpful tips and tricks, see The Sainsbury Lab website or Engler & Marillonet. Golden Gate...technology has adapted Golden Gate cloning for inserting the appropriate oligonucleotides specifying a ...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...secondary antibodies instead (B).   Since a polyclonal primary antibody contains a mix of different...antibodies used for detection bind to non-target immunoglobulins naturally present in the tissue sample. There...antibody fragments to block the endogenous immunoglobulins and reduce the background staining but they...Biological systems tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex...

  5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...distinct proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic domains tethered...are a number of drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that..., Charlton et al. 2020). Most importantly, TET proteins affect transcription independent of their enzymatic...overlap the CpG of interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1...

  6. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...mutation in the evolved TadA domain. Because ABE7.10 contains a heterodimeric fusion of wild-type TadA and TadA7.10... the evolved TadA region. The ABE8.x-m version contains only the evolved TadA and is ~500 base-pair smaller...evaluate further.  The creation of accidental insertions and deletions (indel) by the catalytically impaired...more than 90% compared to ABE7.10. Choosing Cas9 proteins to increase the targeting scope of adenine base... Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: NG) to create NG-ABE8...

  7. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...challenge is finding relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight...search that’s constantly updated. This Twitter list contains >300 Twitter literature bots, including a bot ...AI-backed literature search developed by the Allen Institute for Artificial Intelligence. Semantic Scholar ...Initiative. Meta is not a traditional search engine. Instead, you create your own research feed by entering ... papers are published, similar to Facebook or Instagram. Key features Setting up a personalized feed is...

  8. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... they bind to a class of Argonaute proteins. The Argonaute proteins remove one strand of the siRNA and...cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid backbone. The...the process is a little more complicated. These strains are more picky eaters and refuse to eat liver paste...Sánchez lab protocol also provides step-by-step instructions for the “the bravest and more curious” students...

  9. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...first make your poster. While a poster generally contains the same sections as a primary research article...in your poster. This is a section where you can insert your references and contact information, and it...conclusions, and acknowledgements sections are inserted. The advantage of this style is that it will display... a poster vertically rather than horizontally. Instead of displaying your information as columns, you ... poster, written and graphical information are inserted into larger circles or ovals, that are arranged...

  10. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...use a standard strain, like DH5α, grown at 30 °C instead of 37 °C. If you’re unsure about the integrity ...prefer to store freshly harvested virus at 4 °C instead of storing it briefly at -80 °C. If troubleshooting...option may be to avoid freezing altogether, and instead to use the virus immediately upon harvesting. Interestingly...appear as a white pellet. The pellet can then be rinsed and resuspended in cold sterile PBS.  Alternatively...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with the first...will lead to advances in human health. It was inspiring to hear how the genome project has already had...enzymatic machinery (i.e. RNA replication with no proteins). The chemistry is complex and this work requires...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...system. His lab is also exploring the use of cas9 proteins from different species for genome engineering....

  12. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...—an optical insulator called a ferrule (below). The fiber optic cable/ferrule is inserted into the target...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to...although you may be able to purchase one pre-made instead). Laser stimulation         In the video above...: closed/light receptive, open, closed/light insensitive. If you deliver a continuous stream of photons...

  13. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... sunglasses — these proteins are bright! Most current yellow fluorescent proteins tend to photobleach ...recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et al., 2025)...or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider trying mGold2s...2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications, 16...

  14. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...variance of size of plasmids in your library (Are the inserts of different sizes?), and the desired coverage ...100x coverage of your library, and your library contains 5,000 plasmids, your goal for your transformation...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription factor...library is of a smaller size with a single size insert, liquid media can be faster and easier to work ...protocol paper by the Zhang lab, and the Broad Institute’s general pDNA Library Amplification protocol are...

  15. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...practice: Rehearse your presentation with people inside and outside your field. Your colleagues, lab mates... tack up your poster seconds before the session begins. Plus, you’ll have extra time to practice at the... in to the next figure. Remember your outline: Instead of getting bogged down in the minutia or coming... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...in to thirty-minute-long presentations, which constrains the number of posters you can see. If you are...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...pastoris) because it secretes fewer of its own proteins, making the purification process simpler. Due ... the production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized...SDS-PAGE gel. Once stained, the heavy and light chains become visible alongside your ladder and a protein... density of the antibody bands can be measured against the protein standard and the concentration may ...Any bands on the gel beside the heavy and light chains can also be measured. Contaminating bands lower...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...previous books focused primarily on fluorescent proteins and their applications, tackles much broader issues...from mildly surprising to genuinely shocking. Mainstream media outlets have addressed CRISPR frequently...science produced by the quieter, more reserved Franklins and Prashers will find it increasingly difficult...Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their... Eric J. Perkins...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...sensors. Given the expanding palette of fluorescent proteins and the availability of powerful imaging technologies...modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation signals...combined to create a single polycistronic 15 kb insert. The final construct was then introduced to HeLa...later combine all four constructs into one 15 kb insert. Table 1: MXS Constructs for Labeling Cellular ... fluorescent proteins...

  19. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... for instance, a recent preprint released by the Boehm and Beroukhim labs at the Broad Institute of MIT...MIT and Harvard, Dana-Farber Cancer Institute, and Koch Institute, where an exploration into the effects...introduced an ancestry bias into their experiments instead of a true correlation.   It turned out there was...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher...a similar tool shortly thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same...a more specific A → C base editor (ACBE), they inserted the deaminase into the C-terminal domain of Cas9...excises inosine can be modified to target guanosine instead, perhaps analogous engineering of UNG, which excises...its homologs) has more than one name. Indel Insertion/deletion A type of unwanted DNA edit. AYBE, ...
Showing: 461 - 480 of 842 results