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We narrowed to 763 results for: ire;

Showing: 461 - 480 of 763 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...Brainbow-1.0 in that transient Cre recombination is required to prevent constant inversion of the construct...derivatives of the fluorescent proteins, which are directly trafficked to cell membranes. Membrane trafficking... Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3)...

  2. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...advantage to using direct epigenetic modifiers? As with any experiment, your desired outcome will determine... DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of ...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus. ...indel formation than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ... in a DNA double strand break (DSB), the event required for frameshift and knock-in mutations. If one ...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the...AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene expression. However, it ... As single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression ...

  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...other markers can be injected into an animal, to directly label circulating cells or other cells that are...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...intended application — here, flow cytometry — is the desired target, using one or more of the approaches outlined...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

  9. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...

  10. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D...

  11. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...

  12. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA or RNA sequences...Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular DNA or RNA sequences...

  13. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

  14. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

  15. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

  16. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...postdocs various academic institutions can afford to hire. It will be difficult for some of these institutions...

  17. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard ...
Showing: 461 - 480 of 763 results