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We narrowed to 811 results for: lars

Showing: 461 - 480 of 811 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... you rule out errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading ...important to validate these assumptions for your particular experiment and choice of loading control. First...Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls. These are often called...in many cases, you should validate it for your particular setup. We recommend starting by searching the...

  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...making this system useful for visualization of cellular extensions, such as neurons in the fly visual ...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...classes and resources that can provide details and vocabulary for this skill. What this post may do is help...something should be communicated is necessary in a large organization, because confirming that every person... I don’t just rely on what I know about those particular teams at that given time. I think about how previous... previous iterations of those teams resolved similar issues in the past, or how completely different teams...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... factors of phages) constitute one of the most popular classes of phage proteins to study. These are highly...characterizing sugars and sugar-containing molecules in molecular biology labs (for a review, see Simpson et al.... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends... and a few proteases knocked out making them particularly useful for protein production), then dilute ...

  6. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ... excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   Plasmids...everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The...updated with twelve new sections, including our popular "Dimers and Multimers" post. It's also been re-...

  7. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...Addgene We'll be posting these animations regularly from now until May, so follow us to get updates whenever...

  8. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

    Type
    Blog Post
    Published
    March 9, 2018, 2 p.m.
    ...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX...DNA to read its sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038...

  10. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human...

  11. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... here in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

  12. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...expertise through our blog. Do you have skill in a particular technique? Do you have a set of tips you give... An overview of software tools useful to any molecular biologist Tips for protein purification Replica...Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR...

  13. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...fluorescent markers or protein tags, and suitable for molecular cloning. Plus, we decided to include 10 plasmids...µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such as cloning, digestions... Learn about our other beta projects Read our Molecular Biology Reference ...

  14. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... countries. Guides and protocols were our most popular content, while our beloved blog received 1.3 million...scientists in this rapidly changing environment. In particular, we are working to understand what challenges...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...

  15. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    Published
    July 26, 2018, 12:46 p.m.
    ...phosphorylated on at least one site. Phosphorylation is particularly important for regulating signal transduction...their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a proxy measurement of ... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...like GFP came to be used as research tools is particularly interesting. Although humans have known about...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ...Soon, the number of people studying it grew. In particular, studies in the laboratory of UCSD biochemist...

  17. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...without controversy, and it remains to be seen how popular any one of them will become. One thing is for sure...was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested plasmid from 2016...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety of...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron... to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies...applies naturally occurring bacterial “molecular buoyancy devices” (also known as gas vesicles) as acoustic...

  19. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific ...

  20. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016... perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent...
Showing: 461 - 480 of 811 results