An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
Type
Blog Post
Published
Dec. 17, 2020, 1:30 p.m.
...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals struck me as a “...from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals can be obtained in... in eight days. The only animals on a plate, other than the parental animal, are rescued progeny, which...for any selective marker to remain in the edited animal.
Development of CRISPR/Cas9 editing strategies...must be removed, which can be done by isolating animals with particular visible phenotypes. These approaches...conditional-lethal mutation to identify edits with minimal screening effort. Selecting for repair of a lethal...