Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 855 results for: ple

Showing: 461 - 480 of 855 results

  1. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen, 1992) because of their ... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...beginning to realize that the situation is already complex enough that a deep understanding of retroviral ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions...Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological Reviews, 56(3), 375...

  2. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...unprepared. Each year in the United States, two million people will be infected by antibiotic resistant bacteria...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

  3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...with the viral polymerase, which is formed by a complex of two proteins: the large (L) catalytic protein...-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as ...efficiently reconstitute a functional viral polymerase complex and utilize the minigenome as a template. Thus,...transfected into cells are also extremely important. For example, particular ratios of plasmids encoding NP and ...mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ... a few time-tested tags and fusion proteins as examples. The tables list more common tags than are described...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

  5. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...four-base overhangs flanking each genetic part. (A) Example Level 0 assembly. BpiI recognition sites and four-base... Level 0 acceptor (bottom) for CDS1 parts. (B) Example Level 1 assembly. The choice between different ...important that these stay frozen at -80 °C, since multiple rounds of freezing and thawing will affect bacterial...plasmids with undesired ligations, so always screen multiple colonies after each transformation. This could... MoClo user, but beginners or those performing complex assemblies should screen a minimum of 8–10 colonies...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...easy to share plasmids, both for people depositing plasmids and for people requesting them. Prior to this... and remove these logistical barriers, allowing people to access a wide catalog of plasmids from all over... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism,...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...babble as baby birds, and have dialects just like people. This is actually rare in the animal kingdom. Gasterosteus... most grown crop in the world so it feeds a lot people. This lab is trying to improve homologous recombination...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...citations so that people can go back and read the original reference for the data. For example, we summarized...sense of the themes in the field and to learn what people cared about. I also used reviews to get a list ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...paraphrase what I have heard many other creative people say about writing: you don’t know what it’s going...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity... Rett Syndrome MECP2 follows the “Goldilocks” principle, in which too much of the MECP2 protein is as ...editing can repair a gene in a living animal across multiple cell types using a mouse model for a human neurological...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA... the 20 amino acids and three stop codons. (B) Example of codon usage among different organisms. There...prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth codon optimizing a human gene ...optimized for expression in mammalian cells. For example, Henry Lester’s lab optimized the C. elegans GluCl...wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually be critical for...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ....), while PE6c and PE6d were most efficient at complex edits, such as long or highly structured templates...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...tet-responsive element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...manipulated for use in molecular biology studies. For example the E. coli biotin ligase, BirA, has been used ...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...abilities. The axolotl has the ability to regenerate complex structures such as limbs, the spinal cord, and ...them an ideal model organism; they are relatively simple to maintain and breed and they have large, easily... and microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...cells will be co-transduced with multiple copies of both vectors. Examples of use: Halbert et al., 2002...then following transduction, the partial genomes complement each other to restore the full-expression cassette...outperform single oversized AAVs (Hirsch et al, 2013). Examples of use: Lai et al., 2005 used a trans-splicing... the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a hybrid split...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for...trace file generated in Sanger sequencing and a simple algorithm to quantify editing efficiency by insertion...have to screen to identify a knockout clone. For example, if your cell line is diploid (2N) and your out...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso... Multiple Authors...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...protein phosphorylation in an array of model systems coupled with advances in mass spectrometry suggest that...recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA synthetase... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to... give you a fuller picture. Consider talking to people in the lab across the hall or in the same department...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible.... lab members alone during the interview period. People seem to be hiding their work and are nervous about...successful. Collaborative Competitive Papers have multiple authors One author plus PI on most papers Productive...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...are performing CRISPR library screens, the same principle holds true - the higher the number of distinct...

  18. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...journey is at an end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted... name. So we reached out to you and received a plethora of options, ranging from Affie to Yncredibody....Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this...Original Blugene and Aavery would like your attention, please, as they introduce their new friend…  Fig...

  20. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ...cells are fused with activated B cells from the spleen of mice injected with the antigen of interest. ... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...
Showing: 461 - 480 of 855 results