We narrowed to 867 results for: ple

...specific type of virus you are trying to make. For example, bacteriophages will need to be produced in bacteria...cells. However, some viruses can be produced in multiple different cell types, including plants, mammals...supernatant is typically collected over the course of a couple days to increase yield. The collection process ...preps, lentiviral titration requires an additional couple of days, as it requires transduction of more cells...titrated following purification using qPCR or digital droplet PCR. Insect cells Insect cells are a less common...

...criticism, and ask thought-provoking questions. For example, if you’re a virologist presenting at the American...like to say to help build your presentation. For example, when thinking about discussing a figure, jot down...practice, practice: Rehearse your presentation with people inside and outside your field. Your colleagues,...brainstorm potential questions you may receive. People often ask about the relationship of your work to...guilty of this, and have many times answered a completely different question than the intended one. Take...

...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...like “Oh,…I didn’t quite appreciate that….” For example, when Tom Steitz was presented with a physical ... close to its reported cleavage site on the complementary DNA strand. And this is why we love working ...double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target...

...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...localized to a specific region of the cell. For example, if the ratio of red/green fluorescence falls within...experimental setup less cumbersome. 3. Portability across multiple systems Terskikh et al. showed that their versatile...in gene expression. In a developing embryo, for example, a researcher could determine whether or not newly...

...I knew when I first became a manager. Know your people Each person is unique and should be managed accordingly...accordingly.  I wish I knew that when I was “managing” a couple of undergrads back in grad school. Both were hard-working...Myers-Briggs test:  my undergrads were different people! More specifically, they had different learning... saying “I’ve been doing some of X’s work for a couple of weeks now”. I had no idea this was happening... and maybe even the slight awkwardness can spur people to speak up. Once aware of the problem, I was able...

...using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate...unprepared. Each year in the United States, two million people will be infected by antibiotic resistant bacteria...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

...with the viral polymerase, which is formed by a complex of two proteins: the large (L) catalytic protein...-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as ...efficiently reconstitute a functional viral polymerase complex and utilize the minigenome as a template. Thus,...transfected into cells are also extremely important. For example, particular ratios of plasmids encoding NP and ...mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ...

...vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen, 1992) because of their ... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...beginning to realize that the situation is already complex enough that a deep understanding of retroviral ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions...Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological Reviews, 56(3), 375...

...increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and ... not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is ...lots of individual cells will be transduced with multiple viral particles and labeled a variety of colors...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ... a few time-tested tags and fusion proteins as examples. The tables list more common tags than are described...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

...). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity... Rett Syndrome MECP2 follows the “Goldilocks” principle, in which too much of the MECP2 protein is as ...editing can repair a gene in a living animal across multiple cell types using a mouse model for a human neurological...

...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA... the 20 amino acids and three stop codons. (B) Example of codon usage among different organisms. There...prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth codon optimizing a human gene ...optimized for expression in mammalian cells. For example, Henry Lester’s lab optimized the C. elegans GluCl...wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually be critical for...

...four-base overhangs flanking each genetic part. (A) Example Level 0 assembly. BpiI recognition sites and four-base... Level 0 acceptor (bottom) for CDS1 parts. (B) Example Level 1 assembly. The choice between different ...important that these stay frozen at -80 °C, since multiple rounds of freezing and thawing will affect bacterial...plasmids with undesired ligations, so always screen multiple colonies after each transformation. This could... MoClo user, but beginners or those performing complex assemblies should screen a minimum of 8–10 colonies...

...easy to share plasmids, both for people depositing plasmids and for people requesting them. Prior to this... and remove these logistical barriers, allowing people to access a wide catalog of plasmids from all over... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

....), while PE6c and PE6d were most efficient at complex edits, such as long or highly structured templates...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...tet-responsive element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

...genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism,...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...babble as baby birds, and have dialects just like people. This is actually rare in the animal kingdom. Gasterosteus... most grown crop in the world so it feeds a lot people. This lab is trying to improve homologous recombination...

...citations so that people can go back and read the original reference for the data. For example, we summarized...sense of the themes in the field and to learn what people cared about. I also used reviews to get a list ...original paper if you wanted to learn more. Another example: we made a schematic of all the proteins in the...paraphrase what I have heard many other creative people say about writing: you don’t know what it’s going...

... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for...trace file generated in Sanger sequencing and a simple algorithm to quantify editing efficiency by insertion...have to screen to identify a knockout clone. For example, if your cell line is diploid (2N) and your out...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso... Multiple Authors...

...manipulated for use in molecular biology studies. For example the E. coli biotin ligase, BirA, has been used ...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...abilities. The axolotl has the ability to regenerate complex structures such as limbs, the spinal cord, and ...them an ideal model organism; they are relatively simple to maintain and breed and they have large, easily... and microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles...

...cells will be co-transduced with multiple copies of both vectors. Examples of use: Halbert et al., 2002...then following transduction, the partial genomes complement each other to restore the full-expression cassette...outperform single oversized AAVs (Hirsch et al, 2013). Examples of use: Lai et al., 2005 used a trans-splicing... the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a hybrid split...