... DNA to favor the edit Once the prime editor incorporates the edit into one strand, there’s a mismatch...MutSα–MutLα mismatch repair (MMR) complex, to temporarily inhibit this process and promote copying of the...it from hybridizing with the unedited strand. Temporarily inhibiting this complex may allow time for 5′...

.... They also mutagenized the cytidine deaminase portion of the base editor to create SpCas9 base editors...off-target activity (Richter et al., 2020). This is important because ABEs have generally been slow, meaning...editing for plants Read about four base editor reporters Additional Resources on Addgene.org Catch up...

...the phage recombineering machinery are first electroporated with pCas9. Then, pCRISPR is introduced along... a mammalian cell line (Qi et al., 2013). Two important features of this early system are its regulatable...Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014) Exploiting...

...reflections on the program are here. This was a great opportunity for Addgene to present our own data on plasmid...in advancing this field and how we are able to support rapid progress in all areas of research. We welcome...

...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that...

...India, and Brazil.  We've also created a robust support system for scientists using materials in our repository...repository, from our customer and science support to our efficient and easy tech transfer process to our...

...biology and how it speeds science. Chapter 8 - The importance of communicating research findings through social... singapore...

...Whenever possible, we love to give scientists the opportunity to share their knowledge. One of our goals is...social media outlets meaning you’ll have the opportunity to interact with and discuss your work with many...

... well as plasmids from Addgene to bring new opportunities to Ghanaian scientists Click here to listen... Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end : Kwabena's time in Boston...

...to meet the diverse needs of our community and support a wide range of experimental designs. Here are ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

...synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use viral ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

...repositories, such as Addgene and the DSHB, play an important role in scientific exchange. In addition to ensuring...provide standardized quality-control and technical support to the community. Thanks to Our Guest Blogger! ...

...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ...

... list some easy but effective habits you can incorporate to reduce your lab consumption and increase your...in your daily habits can feel ineffective or unimportant. With so many big contributors to the sustainability...

...out our blog post on CRISPR software to see the importance of programming for scientists!). Careers that...scientist or a professor. There are amazing opportunities that are offered with a biology major.” After...

...~10 years! We're extremely proud to be able to support scientists by bringing you all the plasmids you...storing them, providing quality control and customer support, making plasmids easy for you to find and request...

... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...recommend a few: -The recently established Ebola Genome Portal at the University of California, Santa Cruz, offers...

...water of HR work. What do you think is the most important resource that Addgene provides the scientific ... how to operate during the pandemic, but it’s important to develop a plan for moving forward. Sooner or...

...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...