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We narrowed to 861 results for: ren

Showing: 461 - 480 of 861 results

  1. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...but in time I realized its value. My master’s strengthened my critical thinking and problem solving abilities...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...generalist? Working on a variety of projects and in different fields of research is one of the best parts of...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...RepCap plasmid and its products. Created with BioRender.com   Replication (rep) gene The rep gene encodes... proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins. While ...serotypes are responsible for serotype-specific differences in tissue and/or cell type tropism. Introducing... learn more about the AAV transfer plasmid! References: Naso MF, Tomkowicz B, Perry WL III, Strohl ...

  3. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ...pretend that that there aren’t problems with the academic enterprise or that there weren’t some pretty hefty...the News. While research experience is key to my current position, I think it’s largely my work developing...information distillation, etc) are transferable to many different fields. If you start thinking about preparing ...

  4. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...transposase has been discovered in the genomes of many different fish species in a variety of non-functional forms... vectors were developed into 16 variants with different selection markers (blasticidin, neomycin, puromycin... each contain two SfiI restriction sites with different overhangs depending upon whether they are on the...only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty...

  5. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...pace Constant worry about grants and renewals What is different about non-academia? Scientific publications...interact with scientists just like you What is different about academia? Publishing in peer reviewed journals...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

  6. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your ... and with the right safety considerations, they aren't dangerous for controlled lab usage. Using viral...that you can swap out your virus type to achieve different biological outcomes. For example, some viruses...target cells or organism, some primary cells and differentiated cell types are picky when it comes to transfection...

  7. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...contacting alumni of your lab or university or use your current contacts to ask who they might suggest you speak...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...

  8. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...Khammash lab can also be found here.   References and Resources References Romano E, Baumschlager A, Akmeriç...functional BLADE variants, too. The team tested different linkers between VVD and the DNA-binding domain... Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...

  10. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...of autophagosomes. This approach, however, has inherent limitations because it turns a dynamic process...provide an overview of four autophagy biosensors currently available from Addgene. Rosella autophagy biosensor...wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure...Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo, and Per...

  11. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... the same. Over the next few years genes from different bacterial (and eventually mammalian) species were...ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded in order to store... keeping with our goal of creating a one-stop reference guide for plasmids we've updated and added new...: 24043817. PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary...

  12. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...these experiments because developing embryos are transparent, so it’s easy to watch development in real time...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...Chromophore-assisted laser inactivation in cell biology. Trends in Cell Biology,18(9), 443-450. doi:10.1016/j.tcb...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...diverse range of projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments...yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...

  14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ... that affect Th2 differentiation and activation, using expression of differentiation factors as the screen...they develop rapidly, they are small, and their transparency allows for whole organism imaging. They created...Gottschalk group hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which...

  15. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...scientist, and proud member of Crowdfight. He is currently based in Spain and works on climate change. You...one of the coordinators at Crowdfight. She is currently based in Spain and works in the field of genetics...

  16. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...identifiers (PIDs), a long-lasting way to identify and reference documents, files, or physical reagents. What ...you, we’ll go through examples to highlight the difference between them using a plasmid available through...the lenti-Cas9-VQR plasmid page. Despite their differences, both of these PID formats for lenti-Cas9-VQR...managed by Addgene. How to cite options using the different PID formats The instructions for how to cite any...

  17. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...personal assistant has to deal with. My inbox currently stands at about 65,000 messages and dealing with... lab and safety manager, researcher, adviser, conference planner, and writer.” Connie Cepko was surprised...every graduate student or postdoc may require different types of mentoring. Some mentees prefer lots of...impossible' or useless.” How do you run your lab differently or the same as your adviser did? Tom Ellis merges...

  18. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...variety of educational programs all focused on current questions and experiments in the field of synthetic...adapted from her own MIT curriculum. Drawing on current questions being asked in MIT’s research labs, BioBuilder...exploration, discovery, and collaboration. One module currently under development comes from the Caltech lab of...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...

  19. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...AAV8) enable different patterns of transduction and transgene expression across different cell types (.... Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses...visualizing hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of...

  20. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...
Showing: 461 - 480 of 861 results