We narrowed to 489 results for: vit;

1. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...transient transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase...

2. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...compared. While a higher slope indicates higher sensitivity, linearity is the most important feature of a...

3. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...interact with the air-water interface (AWI) before vitrification, they can be completely denaturated (left) or...

4. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   Published

   March 28, 2024, 1:15 p.m.

   ...IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin alpha...

5. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...Easi-CRISPR (Quadros et al., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then...

6. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated...

7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

8. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...

9. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...

10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...

11. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    Published

    Feb. 8, 2024, 2:15 p.m.

    ...by Cas does not always correlate with catalytic activity at those sites, especially when DNA mismatches...

12. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you may need to optimize the...

13. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...regulation, as long as they can control recombinase activity. For example, post-transcriptional recombinase...

14. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...repeats (ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful...

15. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

16. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...have meetings between teams. This communication is vital because otherwise I’d lose touch with my colleagues...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln...

18. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...

19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low levels...

20. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...When you are hunting for labs to join, one of the vital things to look for is a supportive environment—it...