We narrowed to 783 results for: INA

... Cysteine-less, fast split intein; N-terminal CLm and C-terminal Aes gp41-1 Split inteins A small, fast...interfaces and the freedom to test out lots of combinations, to end up finding something surprisingly useful...attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection HaloTag ...PCV-Cas9, Cas9-PCV HUH-tag Cas9 plasmids with N- or C-terminal PCV2 HUH-tag; increase homology-directed repair...

...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific...rapid means of comparing functionality of N- and C-terminal fusions and degradation in a cell line of interest...express ENL-FKBP12F36V and endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules...express FKBP12F36V-MELK and endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules...

...any type of DNA or RNA purification to avoid contamination, take extra care when performing RNA extraction...using DEPC-treated water, autoclave the water to inactivate the DEPC.  Ensure that plasticware or glassware... baked at 180°C for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...resuspended RNA sample will help ensure DNA contamination is not an issue.  References                 ...

....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...have higher reproducibility and consistency in the final product. Because the constant (non-epitope binding...reduced immunogenicity. Effector functions are eliminated, as is antibody recognition, since antibody-specific...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...

...expressing the recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated...the number of clones that do not contain the recombinant plasmid and therefore makes the cloning process... but should never be used when selecting for recombinant plasmids. Check out our blog post on Common Lab...References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” Biotechniques. 1996 Aug;21(2):320...

...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...solution depended on the specific antibody:target combination of interest (Figure 1). It can therefore be helpful...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...within the five pillars and is frequently used in combination with other approaches. If several cell lines ...correlation with other approaches when possible. Finally, correlation of -omics data can also be used in... 2019, 2021). In those studies, we used this combination to find suitable antibodies for a target with...

...every sweet or toy strewn across the floor. In the final wake it all settles down and the value of each item...of research activity and development since its inaugural year in 2012. That’s just 3 to 4 years ago. It...problems various tools are trying to solve, and finally how we might proceed in the future. When we think...for sgRNA design (by Hsu et al., 2013) used a combination of both empirical and approximate methods. The...online”, “CLI”, and “GUI”. Trends: Online tools dominate the software space but generally rely on less ... active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology (2014). PubMed PMID: ...

...ABEs), consists of 3 components:  a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10...selection strategy (Gaudelli et al., 2017) in combination with a synthetic library of TadA sequences to...cause genome-wide, guide-independent off-target deamination, an important attribute for therapeutic application... JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G ...

...), includes I152L in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and...Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155(I152L), for use with VC155 An improved...mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization ...

...your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted from the ...stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible...opsin variant has slightly different activation/inactivation kinetics. For example, the light-gated cation...to get to the fun stuff: the experiments! In our final post in this primer on optogenetics we’ll delve ...Gutierrez DV, et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate rhodopsin...