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We narrowed to 772 results for: INA

Showing: 241 - 250 of 772 results

  1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...ABEs), consists of 3 components:  a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10...selection strategy (Gaudelli et al., 2017) in combination with a synthetic library of TadA sequences to...cause genome-wide, guide-independent off-target deamination, an important attribute for therapeutic application... JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G ...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...), includes I152L in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and...Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155(I152L), for use with VC155 An improved...mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization ...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted from the ...stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible...opsin variant has slightly different activation/inactivation kinetics. For example, the light-gated cation...to get to the fun stuff: the experiments! In our final post in this primer on optogenetics we’ll delve ...Gutierrez DV, et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate rhodopsin...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors...transiently expressed and binds to target DNA. In the final step of targeted gene knock-out (left), a fragment... the cut site through error-free homologous recombination (HR). Adapted from an image provided by Manuel...they attribute this phenomenon to the presence of terminal proteins capping the ends of linear double-stranded...Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... supernatants are centrifuged and filtered to eliminate any HEK-293T cells. If you titrate your viral ... the viral supernatant in between passages to eliminate dead cells and cellular debris. While adding time...because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus. You can...optimized for your purpose. Making your decision The final decision of which system to use will come down to...). HEK293 cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...

  6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...maintaining heterozygosity is necessary and genetic recombination can potentially cause you to lose track of your...use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially are messed.... They are unable to participate in genetic recombination during meiosis. Also, flies are not viable if...feature of balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, ...the Addgene Blog Methods for Site Specific Recombination in Drosophila Learn about Cre-Lox Resources...

  7. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...variety of institutions recently reported their inability to recapitulate the results of Gao et al 2016 ... Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN .../ssDNA complex in the cell. As proposed in the original article, to achieve greater efficacy, researchers...possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein to load an ssDNA guide... message thus far, based on survey respondents "Final Verdict", is that more time is required for NgAgo...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...drive cannot restore the exact original sequence, it can restore the original protein-coding sequence using...genomic changes impose a fitness cost and are eliminated by natural selection, gene drives distort inheritance...strategies that are robust to human error. Used in combination with standard barrier protocols, they can reduce...RNA-guided gene drives is Cas9 genome editing. Finally, gene drives are an inherently collective technology...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...interfere with their differentiation potential. Finally, retroviruses are prone to insertional mutagenesis...lentiviral vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes...plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes...it’s replication competent, it’s difficult to eliminate the virus from all cells, even after many passages...compared to integrating approaches. These methods eliminate the risk of insertional mutagenesis, the presence... outcome, since not all cell types are easily attainable, or easy to reprogram. Basically, there’s no ...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...Homologous Recombination (HR)? How long should the homology arms be for efficient recombination? A10: The... Cas9n (nickase). Tips for using homologous recombination (HR) Q7: Can you summarize HR template vector...specific mutations or insertions by Homologous Recombination (HR), what is the length of each homology arm...destroy the BbsI sites, a double digest should discriminate between positive and negative clones. Clones... the CRISPR/Cas system with your homologous recombination (HR) template, you could then: Confirm your...
Showing: 241 - 250 of 772 results